Rapid and efficient genetic engineering of both wild type and axenic strains of Dictyostelium discoideum

• Peggy Paschke ,

  Roles Conceptualization, Formal analysis, Investigation, Validation, Visualization, Writing – original draft

  * E-mail: ppaschke@mrc-lmb.cam.ac.uk

  Affiliation MRC Laboratory of Molecular Biology, Cambridge, United Kingdom

  http://orcid.org/0000-0002-6248-5678

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• David A. Knecht,

  Roles Conceptualization, Investigation, Writing – review & editing

  Affiliation Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut, United States of America

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• Augustinas Silale,

  Roles Investigation, Writing – review & editing

  Current address: Department of Biochemistry, University of Oxford, Oxford, United Kingdom.

  Affiliation MRC Laboratory of Molecular Biology, Cambridge, United Kingdom

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• David Traynor,

  Roles Investigation, Writing – review & editing

  Affiliation MRC Laboratory of Molecular Biology, Cambridge, United Kingdom

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• Thomas D. Williams,

  Roles Investigation, Writing – review & editing

  Affiliation MRC Laboratory of Molecular Biology, Cambridge, United Kingdom

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• Peter A. Thomason,

  Roles Investigation, Writing – review & editing

  Affiliation Cancer Research UK Beatson Institute Glasgow, Glasgow, United Kingdom

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• Robert H. Insall,

  Roles Supervision, Writing – review & editing

  Affiliation Cancer Research UK Beatson Institute Glasgow, Glasgow, United Kingdom

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• Jonathan R. Chubb,

  Roles Conceptualization, Investigation, Resources, Writing – review & editing

  Affiliations MRC Laboratory for Molecular Cell Biology, University College London, London, United Kingdom, Department of Cell and Developmental Biology, University College London, London, United Kingdom

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• Robert R. Kay,

  Roles Conceptualization, Investigation, Supervision, Writing – original draft, Writing – review & editing

  Affiliation MRC Laboratory of Molecular Biology, Cambridge, United Kingdom

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• Douwe M. Veltman

  Roles Conceptualization, Investigation, Supervision, Validation, Writing – original draft, Writing – review & editing

  Affiliation MRC Laboratory of Molecular Biology, Cambridge, United Kingdom

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