Optimized application of the secreted Nano-luciferase reporter system using an affinity purification strategy

JingZhe Li; ZhiLan Guo; Takashi Sato; Bo Yuan; YanYan Ma; Dan Qian; JuYing Zhong; MengMeng Jin; Peng Huang; LuYang Che; Yi Wang; Yan Lei; ChangZhen Liu

doi:10.1371/journal.pone.0196617

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Open Access

Peer-reviewed

Research Article

Optimized application of the secreted Nano-luciferase reporter system using an affinity purification strategy

JingZhe Li ,

Contributed equally to this work with: JingZhe Li, ZhiLan Guo

Roles Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Writing – original draft, Writing – review & editing

Affiliations Beijing Key Laboratory of Research of Chinese Medicine on Prevention and Treatment for Major Diseases, Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing, China, Department of Biochemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, Japan
⨯
ZhiLan Guo ,

Contributed equally to this work with: JingZhe Li, ZhiLan Guo

Roles Data curation, Formal analysis

Affiliation Beijing Key Laboratory of Research of Chinese Medicine on Prevention and Treatment for Major Diseases, Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing, China
⨯
Takashi Sato,

Roles Formal analysis, Supervision

Affiliation Department of Biochemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, Japan
⨯
Bo Yuan,

Roles Data curation, Formal analysis

Affiliation Department of Applied Biochemistry, School of Pharmacy, Tokyo University of Pharmacy & Life Sciences, Hachioji, Tokyo, Japan
⨯
YanYan Ma,

Roles Formal analysis

Affiliation Beijing Key Laboratory of Research of Chinese Medicine on Prevention and Treatment for Major Diseases, Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing, China
⨯
Dan Qian,

Roles Formal analysis

Affiliation Beijing Key Laboratory of Research of Chinese Medicine on Prevention and Treatment for Major Diseases, Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing, China
⨯
JuYing Zhong,

Roles Formal analysis

Affiliation Beijing Key Laboratory of Research of Chinese Medicine on Prevention and Treatment for Major Diseases, Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing, China
⨯
MengMeng Jin,

Roles Formal analysis

Affiliation Department of Geriatric Endocrinology, The General Hospital of Chinese People’s Liberation Army, Beijing, China
⨯
Peng Huang,

Roles Funding acquisition

Affiliation Department of Orthopaedics, The General Hospital of Chinese People’s Liberation Army, Beijing, China
⨯
LuYang Che,

Roles Formal analysis

Affiliation Department of Orthopaedics, The General Hospital of Chinese People’s Liberation Army, Beijing, China
⨯
Yi Wang,

Roles Formal analysis

Affiliation Beijing Key Laboratory of Research of Chinese Medicine on Prevention and Treatment for Major Diseases, Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing, China
⨯
Yan Lei ,

Roles Formal analysis

* E-mail: lcz0220@163.com (CZL); leiy999@163.com (YL)

Affiliation Beijing Key Laboratory of Research of Chinese Medicine on Prevention and Treatment for Major Diseases, Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing, China
⨯
ChangZhen Liu

Roles Conceptualization, Funding acquisition, Supervision, Validation, Writing – original draft, Writing – review & editing

* E-mail: lcz0220@163.com (CZL); leiy999@163.com (YL)

Affiliation Beijing Key Laboratory of Research of Chinese Medicine on Prevention and Treatment for Major Diseases, Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing, China

http://orcid.org/0000-0003-1388-4012

⨯

Optimized application of the secreted Nano-luciferase reporter system using an affinity purification strategy

JingZhe Li,
ZhiLan Guo,
Takashi Sato,
Bo Yuan,
YanYan Ma,
Dan Qian,
JuYing Zhong,
MengMeng Jin,
Peng Huang,
LuYang Che

Published: May 2, 2018
https://doi.org/10.1371/journal.pone.0196617

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