Influence of the fixation/permeabilization step on peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) for the detection of bacteria

• Rui Rocha ,

  Contributed equally to this work with: Rui Rocha, Carina Almeida, Nuno F. Azevedo

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Visualization, Writing – original draft, Writing – review & editing

  * E-mail: pdeqb1209967@fe.up.pt

  Affiliations LEPABE—Laboratory for Process Engineering, Environment, Biotechnology and Energy, Department of Chemical Engineering, Faculty of Engineering of the University of Porto, Porto, Portugal, CEB—Centre of Biological Engineering, University of Minho, Braga, Portugal, BIOMODE—Biomolecular Determination S.A., Braga, Portugal

  http://orcid.org/0000-0003-3859-2702

  ⨯
• Carina Almeida ,

  Contributed equally to this work with: Rui Rocha, Carina Almeida, Nuno F. Azevedo

  Roles Conceptualization, Formal analysis, Funding acquisition, Investigation, Methodology, Supervision, Validation, Writing – review & editing

  Affiliations LEPABE—Laboratory for Process Engineering, Environment, Biotechnology and Energy, Department of Chemical Engineering, Faculty of Engineering of the University of Porto, Porto, Portugal, CEB—Centre of Biological Engineering, University of Minho, Braga, Portugal, BIOMODE—Biomolecular Determination S.A., Braga, Portugal, INIAV, IP- National Institute for Agrarian and Veterinary Research, Rua dos Lagidos, Lugar da Madalena, Vairão, Vila do Conde, Portugal

  ⨯
• Nuno F. Azevedo

  Contributed equally to this work with: Rui Rocha, Carina Almeida, Nuno F. Azevedo

  Roles Conceptualization, Funding acquisition, Supervision, Validation, Writing – review & editing

  Affiliation LEPABE—Laboratory for Process Engineering, Environment, Biotechnology and Energy, Department of Chemical Engineering, Faculty of Engineering of the University of Porto, Porto, Portugal

  ⨯