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Open Access

Peer-reviewed

Research Article

Rapid detection of Puccinia triticina causing leaf rust of wheat by PCR and loop mediated isothermal amplification

C. Manjunatha ,

Contributed equally to this work with: C. Manjunatha, Sapna Sharma, Rashmi Aggarwal

Roles Data curation, Formal analysis, Investigation, Methodology, Resources, Validation, Visualization, Writing – original draft, Writing – review & editing

Current address: ICAR—Indian Agricultural Research Institute, Regional Station, Wellington, The Nilgiris, Tamil Nadu, India

Affiliation Fungal Molecular Biology Laboratory, Division of Plant Pathology, ICAR—Indian Agricultural Research Institute, New Delhi, India
⨯
Sapna Sharma ,

Contributed equally to this work with: C. Manjunatha, Sapna Sharma, Rashmi Aggarwal

Roles Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – original draft, Writing – review & editing

Affiliation Fungal Molecular Biology Laboratory, Division of Plant Pathology, ICAR—Indian Agricultural Research Institute, New Delhi, India
⨯
Deepika Kulshreshtha,

Roles Formal analysis, Investigation, Validation

Affiliation Fungal Molecular Biology Laboratory, Division of Plant Pathology, ICAR—Indian Agricultural Research Institute, New Delhi, India
⨯
Sangeeta Gupta,

Roles Formal analysis, Methodology, Validation

Affiliation Fungal Molecular Biology Laboratory, Division of Plant Pathology, ICAR—Indian Agricultural Research Institute, New Delhi, India
⨯
Kartar Singh,

Roles Formal analysis, Investigation, Validation

Affiliation ICAR-National Bureau of Plant Genetic Resources, New Delhi, India
⨯
Subhash C. Bhardwaj,

Roles Resources

Affiliation ICAR—Indian Institute of Wheat and Barley Research, Regional Station, Flowerdale, Shimla, India
⨯
Rashmi Aggarwal

Contributed equally to this work with: C. Manjunatha, Sapna Sharma, Rashmi Aggarwal

Roles Conceptualization, Data curation, Funding acquisition, Project administration, Resources, Supervision, Writing – review & editing

* E-mail: rashmi.aggarwal2@gmail.com

Affiliation Fungal Molecular Biology Laboratory, Division of Plant Pathology, ICAR—Indian Agricultural Research Institute, New Delhi, India

http://orcid.org/0000-0002-8706-6680

⨯

Rapid detection of Puccinia triticina causing leaf rust of wheat by PCR and loop mediated isothermal amplification

C. Manjunatha,
Sapna Sharma,
Deepika Kulshreshtha,
Sangeeta Gupta,
Kartar Singh,
Subhash C. Bhardwaj,
Rashmi Aggarwal

x

Published: April 26, 2018
https://doi.org/10.1371/journal.pone.0196409

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Wheat
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Polymerase chain reaction
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Leaves
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Plant pathogens
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Genomics
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Colorimetric assays
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Fungal pathogens
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Gel electrophoresis
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