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Open Access
Peer-reviewed
Research Article
Versatile approach for functional analysis of human proteins and efficient stable cell line generation using FLP-mediated recombination system
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Roman J. Szczesny ,
Roles Conceptualization, Funding acquisition, Investigation, Methodology, Supervision, Visualization, Writing – original draft, Writing – review & editing
* E-mail: rszczesny@ibb.waw.pl (RJS); andrzejd@ibb.waw.pl (AD)
Affiliations Laboratory of RNA Biology and Functional Genomics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland, Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, Warsaw, Poland
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Katarzyna Kowalska,
Roles Investigation, Methodology, Visualization
Affiliation Laboratory of RNA Biology and Functional Genomics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland
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Kamila Klosowska-Kosicka,
Roles Investigation, Methodology
Affiliation Laboratory of RNA Biology and Functional Genomics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland
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Aleksander Chlebowski,
Roles Investigation, Methodology, Visualization, Writing – review & editing
Affiliations Laboratory of RNA Biology and Functional Genomics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland, Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, Warsaw, Poland
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Ewelina P. Owczarek,
Roles Investigation, Methodology, Visualization
Affiliation Laboratory of RNA Biology and Functional Genomics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland
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Zbigniew Warkocki,
Roles Funding acquisition, Methodology
Affiliation Laboratory of RNA Biology and Functional Genomics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland
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Tomasz M. Kulinski,
Roles Data curation, Software, Visualization
Affiliation Laboratory of RNA Biology and Functional Genomics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland
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Dorota Adamska,
Roles Data curation, Methodology
Affiliation Laboratory of RNA Biology and Functional Genomics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland
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Kamila Affek,
Roles Investigation
Affiliation Laboratory of RNA Biology and Functional Genomics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland
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Agata Jedroszkowiak,
Roles Investigation, Methodology
Affiliation Laboratory of RNA Biology and Functional Genomics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland
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Anna V. Kotrys,
Roles Investigation
Affiliation Laboratory of RNA Biology and Functional Genomics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland
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Rafal Tomecki,
Roles Methodology
Affiliations Laboratory of RNA Biology and Functional Genomics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland, Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, Warsaw, Poland
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Pawel S. Krawczyk,
Roles Data curation, Software
Affiliation Laboratory of RNA Biology and Functional Genomics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland
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Lukasz S. Borowski,
Roles Investigation, Methodology
Affiliations Laboratory of RNA Biology and Functional Genomics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland, Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, Warsaw, Poland
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Andrzej Dziembowski
Roles Conceptualization, Funding acquisition, Supervision, Writing – review & editing
* E-mail: rszczesny@ibb.waw.pl (RJS); andrzejd@ibb.waw.pl (AD)
Affiliations Laboratory of RNA Biology and Functional Genomics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland, Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, Warsaw, Poland
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Versatile approach for functional analysis of human proteins and efficient stable cell line generation using FLP-mediated recombination system
- Roman J. Szczesny,
- Katarzyna Kowalska,
- Kamila Klosowska-Kosicka,
- Aleksander Chlebowski,
- Ewelina P. Owczarek,
- Zbigniew Warkocki,
- Tomasz M. Kulinski,
- Dorota Adamska,
- Kamila Affek,
- Agata Jedroszkowiak
- Published: March 28, 2018
- https://doi.org/10.1371/journal.pone.0194887
- See the preprint