Development of an improved RT-qPCR Assay for detection of Japanese encephalitis virus (JEV) RNA including a systematic review and comprehensive comparison with published methods

• Tehmina Bharucha ,

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Project administration, Resources, Software, Writing – original draft, Writing – review & editing

  * E-mail: t.bharucha@doctors.org.uk

  Affiliations Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao P.D.R, Division of Infection and Immunity, University College London, London, United Kingdom, Departments of Infectious Diseases and Microbiology, Royal Free Hospital, London, United Kingdom

  http://orcid.org/0000-0002-6772-2855

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• Onanong Sengvilaipaseuth,

  Roles Investigation, Project administration, Resources, Writing – review & editing

  Affiliation Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao P.D.R

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• Manivanh Vongsouvath,

  Roles Project administration, Writing – review & editing

  Affiliation Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao P.D.R

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• Malavanh Vongsouvath,

  Roles Investigation, Resources, Writing – review & editing

  Affiliation Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao P.D.R

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• Viengmon Davong,

  Roles Project administration, Resources, Writing – review & editing

  Affiliation Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao P.D.R

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• Phonepasith Panyanouvong,

  Roles Investigation, Project administration, Writing – review & editing

  Affiliation Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao P.D.R

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• Géraldine Piorkowski,

  Roles Investigation, Writing – review & editing

  Affiliation UMR "Unité des Virus Emergents" (UVE: Aix-Marseille Univ–IRD 190 –Inserm 1207 –IHU Méditerranée Infection), Marseille, France

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• Jeremy A. Garson,

  Roles Conceptualization, Formal analysis, Investigation, Methodology, Software, Supervision, Writing – review & editing

  Affiliations Division of Infection and Immunity, University College London, London, United Kingdom, National Transfusion Microbiology Laboratories, NHS Blood and Transplant, London, United Kingdom

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• Paul N. Newton,

  Roles Conceptualization, Project administration, Resources, Supervision, Writing – review & editing

  Affiliations Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao P.D.R, Centre for Tropical Medicine and Global Health, Nuffield Department of Clinical Medicine, University of Oxford, Churchill Hospital, Oxford, United Kingdom, London School of Hygiene and Tropical Medicine (LSHTM), London, United Kingdom

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• Xavier de Lamballerie,

  Roles Methodology, Supervision, Writing – review & editing

  Affiliation UMR "Unité des Virus Emergents" (UVE: Aix-Marseille Univ–IRD 190 –Inserm 1207 –IHU Méditerranée Infection), Marseille, France

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• Audrey Dubot-Pérès

  Roles Conceptualization, Funding acquisition, Investigation, Methodology, Project administration, Resources, Software, Supervision, Writing – review & editing

  Affiliations Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao P.D.R, UMR "Unité des Virus Emergents" (UVE: Aix-Marseille Univ–IRD 190 –Inserm 1207 –IHU Méditerranée Infection), Marseille, France, National Transfusion Microbiology Laboratories, NHS Blood and Transplant, London, United Kingdom

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