A FRET-based biosensor for measuring Gα13 activation in single cells

Marieke Mastop; Nathalie R. Reinhard; Cristiane R. Zuconelli; Fenna Terwey; Theodorus W. J. Gadella Jr.; Jakobus van Unen; Merel J. W. Adjobo-Hermans; Joachim Goedhart

doi:10.1371/journal.pone.0193705

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Open Access

Peer-reviewed

Research Article

A FRET-based biosensor for measuring Gα13 activation in single cells

Marieke Mastop,

Roles Conceptualization, Investigation, Methodology, Writing – original draft

Affiliation Swammerdam Institute for Life Sciences, Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, University of Amsterdam, Amsterdam, The Netherlands
⨯
Nathalie R. Reinhard,

Roles Investigation, Methodology

Affiliation Swammerdam Institute for Life Sciences, Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, University of Amsterdam, Amsterdam, The Netherlands
⨯
Cristiane R. Zuconelli,

Roles Formal analysis, Investigation

Affiliation Department of Biochemistry, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, The Netherlands
⨯
Fenna Terwey,

Roles Investigation, Methodology

Affiliation Swammerdam Institute for Life Sciences, Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, University of Amsterdam, Amsterdam, The Netherlands
⨯
Theodorus W. J. Gadella Jr.,

Roles Funding acquisition, Supervision

Affiliation Swammerdam Institute for Life Sciences, Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, University of Amsterdam, Amsterdam, The Netherlands
⨯
Jakobus van Unen,

Roles Conceptualization, Investigation, Methodology

Current address: Department of Biology, Institute of Cell Biology, University of Bern, Bern, Switzerland.

Affiliation Swammerdam Institute for Life Sciences, Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, University of Amsterdam, Amsterdam, The Netherlands
⨯
Merel J. W. Adjobo-Hermans,

Roles Formal analysis, Investigation, Methodology

Affiliation Department of Biochemistry, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, The Netherlands
⨯
Joachim Goedhart

Roles Conceptualization, Funding acquisition, Methodology, Supervision, Writing – original draft, Writing – review & editing

* E-mail: j.goedhart@uva.nl

Affiliation Swammerdam Institute for Life Sciences, Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, University of Amsterdam, Amsterdam, The Netherlands

http://orcid.org/0000-0002-0630-3825

⨯

A FRET-based biosensor for measuring Gα13 activation in single cells

Marieke Mastop,
Nathalie R. Reinhard,
Cristiane R. Zuconelli,
Fenna Terwey,
Theodorus W. J. Gadella Jr.,
Jakobus van Unen,
Merel J. W. Adjobo-Hermans,
Joachim Goedhart

Published: March 5, 2018
https://doi.org/10.1371/journal.pone.0193705

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G protein coupled receptors
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