Cell-free synthesis of functional antibody fragments to provide a structural basis for antibody–antigen interaction

• Takayoshi Matsuda ,

  Contributed equally to this work with: Takayoshi Matsuda, Takuhiro Ito

  Roles Conceptualization, Investigation, Methodology, Validation, Visualization, Writing – original draft, Writing – review & editing

  Affiliation Division of Structural and Synthetic Biology, RIKEN Center for Life Science Technologies, 1-7-22 Suehiro-cho, Tsurumiku, Yokohama, Japan

  http://orcid.org/0000-0002-6746-7639

  ⨯
• Takuhiro Ito ,

  Contributed equally to this work with: Takayoshi Matsuda, Takuhiro Ito

  Roles Conceptualization, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – original draft, Writing – review & editing

  Affiliation Division of Structural and Synthetic Biology, RIKEN Center for Life Science Technologies, 1-7-22 Suehiro-cho, Tsurumiku, Yokohama, Japan

  ⨯
• Chie Takemoto,

  Roles Conceptualization, Investigation, Methodology, Project administration, Writing – original draft, Writing – review & editing

  Affiliation Division of Structural and Synthetic Biology, RIKEN Center for Life Science Technologies, 1-7-22 Suehiro-cho, Tsurumiku, Yokohama, Japan

  ⨯
• Kazushige Katsura,

  Roles Investigation, Resources

  Affiliations Division of Structural and Synthetic Biology, RIKEN Center for Life Science Technologies, 1-7-22 Suehiro-cho, Tsurumiku, Yokohama, Japan, RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumiku, Yokohama, Japan

  ⨯
• Mariko Ikeda,

  Roles Investigation, Resources

  Affiliations Division of Structural and Synthetic Biology, RIKEN Center for Life Science Technologies, 1-7-22 Suehiro-cho, Tsurumiku, Yokohama, Japan, RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumiku, Yokohama, Japan

  ⨯
• Motoaki Wakiyama,

  Roles Investigation, Resources

  Affiliations Division of Structural and Synthetic Biology, RIKEN Center for Life Science Technologies, 1-7-22 Suehiro-cho, Tsurumiku, Yokohama, Japan, RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumiku, Yokohama, Japan

  ⨯
• Mutsuko Kukimoto-Niino,

  Roles Investigation, Resources

  Affiliations Division of Structural and Synthetic Biology, RIKEN Center for Life Science Technologies, 1-7-22 Suehiro-cho, Tsurumiku, Yokohama, Japan, RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumiku, Yokohama, Japan

  ⨯
• Shigeyuki Yokoyama,

  Roles Conceptualization, Funding acquisition

  Affiliations RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumiku, Yokohama, Japan, RIKEN Structural Biology Laboratory, 1-7-22 Suehiro-cho, Tsurumiku, Yokohama, Japan

  ⨯
• Yoshikazu Kurosawa,

  Roles Conceptualization, Resources

  Affiliation Innovation Center for Advanced Medicine, Fujita Health University School of Medicine, Toyoake, Aichi, Japan

  ⨯
• Mikako Shirouzu

  Roles Conceptualization, Funding acquisition, Project administration, Supervision, Writing – review & editing

  * E-mail: mikako.shirouzu@riken.jp

  Affiliations Division of Structural and Synthetic Biology, RIKEN Center for Life Science Technologies, 1-7-22 Suehiro-cho, Tsurumiku, Yokohama, Japan, RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumiku, Yokohama, Japan

  ⨯