Ex vivo expansion of alveolar macrophages with Mycobacterium tuberculosis from the resected lungs of patients with pulmonary tuberculosis

Elena Ufimtseva; Natalya Eremeeva; Ekaterina Petrunina; Tatiana Umpeleva; Svetlana Karskanova; Sergey Bayborodin; Diana Vakhrusheva; Marionella Kravchenko; Sergey Skornyakov

doi:10.1371/journal.pone.0191918

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Open Access

Peer-reviewed

Research Article

Ex vivo expansion of alveolar macrophages with Mycobacterium tuberculosis from the resected lungs of patients with pulmonary tuberculosis

Elena Ufimtseva ,

Contributed equally to this work with: Elena Ufimtseva, Natalya Eremeeva

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing

* E-mail: ufim1@ngs.ru

Affiliations Laboratory of Medical Biotechnology, Research Institute of Biochemistry, Novosibirsk, Russia, Scientific Department, Ural Research Institute for Phthisiopulmonology, National Medical Research Center of Tuberculosis and Infectious Diseases of Ministry of Health of the Russian Federation, Yekaterinburg, Russia
⨯
Natalya Eremeeva ,

Contributed equally to this work with: Elena Ufimtseva, Natalya Eremeeva

Roles Investigation, Methodology, Validation

Affiliation Scientific Department, Ural Research Institute for Phthisiopulmonology, National Medical Research Center of Tuberculosis and Infectious Diseases of Ministry of Health of the Russian Federation, Yekaterinburg, Russia
⨯
Ekaterina Petrunina,

Roles Investigation

Affiliation Scientific Department, Ural Research Institute for Phthisiopulmonology, National Medical Research Center of Tuberculosis and Infectious Diseases of Ministry of Health of the Russian Federation, Yekaterinburg, Russia
⨯
Tatiana Umpeleva,

Roles Investigation

Affiliation Scientific Department, Ural Research Institute for Phthisiopulmonology, National Medical Research Center of Tuberculosis and Infectious Diseases of Ministry of Health of the Russian Federation, Yekaterinburg, Russia
⨯
Svetlana Karskanova,

Roles Investigation

Affiliation Scientific Department, Ural Research Institute for Phthisiopulmonology, National Medical Research Center of Tuberculosis and Infectious Diseases of Ministry of Health of the Russian Federation, Yekaterinburg, Russia
⨯
Sergey Bayborodin,

Roles Visualization

Affiliation Shared Center for Microscopic Analysis of Biological Objects, Federal Research Center Institute of Cytology and Genetics, Novosibirsk, Russia
⨯
Diana Vakhrusheva,

Roles Validation

Affiliation Scientific Department, Ural Research Institute for Phthisiopulmonology, National Medical Research Center of Tuberculosis and Infectious Diseases of Ministry of Health of the Russian Federation, Yekaterinburg, Russia
⨯
Marionella Kravchenko,

Roles Resources, Validation

Affiliation Scientific Department, Ural Research Institute for Phthisiopulmonology, National Medical Research Center of Tuberculosis and Infectious Diseases of Ministry of Health of the Russian Federation, Yekaterinburg, Russia
⨯
Sergey Skornyakov

Roles Methodology, Supervision, Validation

Affiliation Scientific Department, Ural Research Institute for Phthisiopulmonology, National Medical Research Center of Tuberculosis and Infectious Diseases of Ministry of Health of the Russian Federation, Yekaterinburg, Russia
⨯

Ex vivo expansion of alveolar macrophages with Mycobacterium tuberculosis from the resected lungs of patients with pulmonary tuberculosis

Elena Ufimtseva,
Natalya Eremeeva,
Ekaterina Petrunina,
Tatiana Umpeleva,
Svetlana Karskanova,
Sergey Bayborodin,
Diana Vakhrusheva,
Marionella Kravchenko,
Sergey Skornyakov

Published: February 5, 2018
https://doi.org/10.1371/journal.pone.0191918

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Mycobacterium tuberculosis
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Alveolar macrophages
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Tuberculosis
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Lung resection
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Surgical and invasive medical procedures
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Histology
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Cell staining
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Lesions
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