Target discovery screens using pooled shRNA libraries and next-generation sequencing: A model workflow and analytical algorithm

• Christiane Schaefer ,

  Contributed equally to this work with: Christiane Schaefer, Nikhil Mallela

  Roles Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – review & editing

  Current address: Department of Hematology and Oncology, Pediatrics III, West German Cancer Center, German Cancer Consortium (DKTK) Center Essen, University Hospital Essen, Essen, Germany

  Affiliation Pediatric Hematology and Oncology, University Hospital Münster, Münster, Germany

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• Nikhil Mallela ,

  Contributed equally to this work with: Christiane Schaefer, Nikhil Mallela

  Roles Data curation, Formal analysis, Investigation, Methodology, Software, Validation, Visualization, Writing – review & editing

  Affiliation Institute of Bioinformatics, Westfälische Wilhelms-Universität Münster, Münster, Germany

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• Jochen Seggewiß,

  Roles Investigation, Methodology, Validation, Writing – review & editing

  Affiliation Institute of Human Genetics, University Hospital Münster, Münster, Germany

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• Birgit Lechtape,

  Roles Data curation, Investigation, Methodology

  Current address: Department of Hematology and Oncology, Pediatrics III, West German Cancer Center, German Cancer Consortium (DKTK) Center Essen, University Hospital Essen, Essen, Germany

  Affiliation Pediatric Hematology and Oncology, University Hospital Münster, Münster, Germany

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• Heymut Omran,

  Roles Supervision

  Affiliation General Pediatrics, University Hospital Münster, Münster, Germany

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• Uta Dirksen,

  Roles Conceptualization, Funding acquisition, Project administration, Resources, Supervision, Validation, Writing – review & editing

  Affiliation Department of Hematology and Oncology, Pediatrics III, West German Cancer Center, German Cancer Consortium (DKTK) Center Essen, University Hospital Essen, Essen, Germany

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• Eberhard Korsching,

  Roles Data curation, Formal analysis, Funding acquisition, Methodology, Project administration, Resources, Software, Supervision, Validation, Writing – review & editing

  Affiliation Institute of Bioinformatics, Westfälische Wilhelms-Universität Münster, Münster, Germany

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• Jenny Potratz

  Roles Conceptualization, Formal analysis, Funding acquisition, Methodology, Project administration, Resources, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing

  * E-mail: jenny.potratz@ukmuenster.de

  Affiliations Pediatric Hematology and Oncology, University Hospital Münster, Münster, Germany, General Pediatrics, University Hospital Münster, Münster, Germany

  http://orcid.org/0000-0002-1327-5981

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