Selection of suitable reference genes for normalization of quantitative RT-PCR (RT-qPCR) expression data across twelve tissues of riverine buffaloes (Bubalus bubalis)

Ramneek Kaur; Monika Sodhi; Ankita Sharma; Vijay Lakshmi Sharma; Preeti Verma; Shelesh Kumar Swami; Parvesh Kumari; Manishi Mukesh

doi:10.1371/journal.pone.0191558

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Open Access

Peer-reviewed

Research Article

Selection of suitable reference genes for normalization of quantitative RT-PCR (RT-qPCR) expression data across twelve tissues of riverine buffaloes (Bubalus bubalis)

Ramneek Kaur,

Roles Data curation, Formal analysis, Writing – original draft, Writing – review & editing

Affiliations Department of Animal Biotechnology, ICAR-National Bureau of Animal Genetic Resources, Karnal, Haryana, India, Department of Zoology, Panjab University, Chandigarh, India
⨯
Monika Sodhi,

Roles Conceptualization, Resources, Writing – original draft, Writing – review & editing

Affiliation Department of Animal Biotechnology, ICAR-National Bureau of Animal Genetic Resources, Karnal, Haryana, India
⨯
Ankita Sharma,

Roles Data curation, Formal analysis, Writing – original draft, Writing – review & editing

Affiliation Department of Animal Biotechnology, ICAR-National Bureau of Animal Genetic Resources, Karnal, Haryana, India
⨯
Vijay Lakshmi Sharma,

Roles Conceptualization, Resources

Affiliation Department of Zoology, Panjab University, Chandigarh, India
⨯
Preeti Verma,

Roles Data curation

Affiliation Department of Animal Biotechnology, ICAR-National Bureau of Animal Genetic Resources, Karnal, Haryana, India
⨯
Shelesh Kumar Swami,

Roles Data curation

Affiliation Department of Animal Biotechnology, ICAR-National Bureau of Animal Genetic Resources, Karnal, Haryana, India
⨯
Parvesh Kumari,

Roles Data curation, Resources

Affiliation Department of Animal Biotechnology, ICAR-National Bureau of Animal Genetic Resources, Karnal, Haryana, India
⨯
Manishi Mukesh

Roles Conceptualization, Formal analysis, Writing – original draft, Writing – review & editing

* E-mail: mmukesh_26@hotmail.com, mmukesh26@gmail.com

Affiliation Department of Animal Biotechnology, ICAR-National Bureau of Animal Genetic Resources, Karnal, Haryana, India

http://orcid.org/0000-0001-8101-3863

⨯

Selection of suitable reference genes for normalization of quantitative RT-PCR (RT-qPCR) expression data across twelve tissues of riverine buffaloes (Bubalus bubalis)

Ramneek Kaur,
Monika Sodhi,
Ankita Sharma,
Vijay Lakshmi Sharma,
Preeti Verma,
Shelesh Kumar Swami,
Parvesh Kumari,
Manishi Mukesh

Published: March 6, 2018
https://doi.org/10.1371/journal.pone.0191558

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Gene expression
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