Minimal 2'-O-methyl phosphorothioate linkage modification pattern of synthetic guide RNAs for increased stability and efficient CRISPR-Cas9 gene editing avoiding cellular toxicity

• Megan Basila,

  Roles Data curation, Formal analysis, Investigation, Methodology, Writing – original draft

  Affiliation Dharmacon, a Horizon Discovery Group company, Lafayette, Colorado, United States of America

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• Melissa L. Kelley,

  Roles Supervision, Writing – review & editing

  Affiliation Dharmacon, a Horizon Discovery Group company, Lafayette, Colorado, United States of America

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• Anja van Brabant Smith

  Roles Writing – review & editing

  * E-mail: anja.smith@horizondiscovery.com

  Affiliation Dharmacon, a Horizon Discovery Group company, Lafayette, Colorado, United States of America

  http://orcid.org/0000-0003-3145-6599

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