Single molecule sequencing of the M13 virus genome without amplification

Luyang Zhao; Liwei Deng; Gailing Li; Huan Jin; Jinsen Cai; Huan Shang; Yan Li; Haomin Wu; Weibin Xu; Lidong Zeng; Renli Zhang; Huan Zhao; Ping Wu; Zhiliang Zhou; Jiao Zheng; Pierre Ezanno; Andrew X. Yang; Qin Yan; Michael W. Deem; Jiankui He

doi:10.1371/journal.pone.0188181

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Open Access

Peer-reviewed

Research Article

Single molecule sequencing of the M13 virus genome without amplification

Luyang Zhao,

Roles Conceptualization, Data curation, Writing – original draft, Writing – review & editing

Affiliation Direct Genomics Co., Ltd., Shenzhen, Guangdong, China
⨯
Liwei Deng,

Roles Investigation, Methodology

Affiliation Direct Genomics Co., Ltd., Shenzhen, Guangdong, China
⨯
Gailing Li,

Roles Investigation

Affiliation Direct Genomics Co., Ltd., Shenzhen, Guangdong, China
⨯
Huan Jin,

Roles Formal analysis, Software

Affiliation Direct Genomics Co., Ltd., Shenzhen, Guangdong, China
⨯
Jinsen Cai,

Roles Investigation

Affiliation Direct Genomics Co., Ltd., Shenzhen, Guangdong, China
⨯
Huan Shang,

Roles Investigation

Affiliation Direct Genomics Co., Ltd., Shenzhen, Guangdong, China
⨯
Yan Li,

Roles Investigation

Affiliation Direct Genomics Co., Ltd., Shenzhen, Guangdong, China
⨯
Haomin Wu,

Roles Investigation

Affiliation Direct Genomics Co., Ltd., Shenzhen, Guangdong, China
⨯
Weibin Xu,

Roles Formal analysis, Software

Affiliation Direct Genomics Co., Ltd., Shenzhen, Guangdong, China
⨯
Lidong Zeng,

Roles Formal analysis, Software

Affiliation Direct Genomics Co., Ltd., Shenzhen, Guangdong, China
⨯
Renli Zhang,

Roles Validation

Affiliation Reproductive Medical Center of Guangdong General Hospital & Guangdong Academy of Medical Sciences, Guangzhou, China
⨯
Huan Zhao,

Roles Validation

Affiliation Shenzhen Armed Police Hospital Reproductive Center, Luohu District, Shenzhen, China
⨯
Ping Wu,

Roles Methodology

Affiliation Direct Genomics Co., Ltd., Shenzhen, Guangdong, China
⨯
Zhiliang Zhou,

Roles Methodology

Affiliation Direct Genomics Co., Ltd., Shenzhen, Guangdong, China
⨯
Jiao Zheng,

Roles Resources

Affiliation Direct Genomics Co., Ltd., Shenzhen, Guangdong, China
⨯
Pierre Ezanno,

Roles Resources

Affiliation Direct Genomics Co., Ltd., Shenzhen, Guangdong, China
⨯
Andrew X. Yang,

Roles Writing – review & editing

Affiliation Department of Biology, South University of Science and Technology of China, Shenzhen, Guangdong, China
⨯
Qin Yan,

Roles Project administration

Affiliation Direct Genomics Co., Ltd., Shenzhen, Guangdong, China
⨯
Michael W. Deem,

Roles Writing – review & editing

Affiliation Departments of Bioengineering and Physics & Astronomy, Rice University, Houston, TX, United States of America
⨯
[ ... ],
Jiankui He

Roles Conceptualization, Data curation, Funding acquisition, Project administration, Writing – original draft, Writing – review & editing

* E-mail: hejk@sustc.edu.cn

Affiliation Department of Biology, South University of Science and Technology of China, Shenzhen, Guangdong, China
⨯
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Single molecule sequencing of the M13 virus genome without amplification

Luyang Zhao,
Liwei Deng,
Gailing Li,
Huan Jin,
Jinsen Cai,
Huan Shang,
Yan Li,
Haomin Wu,
Weibin Xu,
Lidong Zeng

Published: December 18, 2017
https://doi.org/10.1371/journal.pone.0188181
See the preprint

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12 Jul 2019
16:17 GMT
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Author udpate Posted by mwdeem on 12 Jul 2019 at 16:17 GMT

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Next-generation sequencing
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Vector cloning
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Sequence alignment
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