Steps to achieve quantitative measurements of microRNA using two step droplet digital PCR

• Erica V. Stein ,

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Project administration, Validation, Writing – original draft, Writing – review & editing

  * E-mail: Erica.Stein@nist.gov

  Affiliation Biosystems and Biomaterials Division, Materials Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland, United States of America

  http://orcid.org/0000-0001-8778-8846

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• David L. Duewer,

  Roles Formal analysis, Methodology, Visualization, Writing – review & editing

  Affiliation Chemical Sciences Division, Materials Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland, United States of America

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• Natalia Farkas,

  Roles Formal analysis, Investigation, Methodology, Writing – review & editing

  Affiliation Engineering Physics Division, Physical Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland, United States of America

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• Erica L. Romsos,

  Roles Investigation, Methodology, Writing – review & editing

  Affiliation Biomolecular Measurement Division, Materials Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland, United States of America

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• Lili Wang,

  Roles Methodology, Resources, Supervision, Writing – review & editing

  Affiliation Biosystems and Biomaterials Division, Materials Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland, United States of America

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• Kenneth D. Cole

  Roles Conceptualization, Methodology, Resources, Supervision, Writing – review & editing

  Affiliation Biosystems and Biomaterials Division, Materials Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland, United States of America

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