Identification of two immortalized cell lines, ECV304 and bEnd3, for in vitro permeability studies of blood-brain barrier

Shu Yang; Shenghui Mei; Hong Jin; Bin Zhu; Yue Tian; Jiping Huo; Xu Cui; Anchen Guo; Zhigang Zhao

doi:10.1371/journal.pone.0187017

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Open Access

Peer-reviewed

Research Article

Identification of two immortalized cell lines, ECV304 and bEnd3, for in vitro permeability studies of blood-brain barrier

Shu Yang,

Roles Conceptualization, Data curation, Investigation, Methodology, Writing – original draft

Affiliation Department of Pharmacy, Beijing Tiantan Hospital, Capital Medical University, Beijing, China

http://orcid.org/0000-0003-4755-5348

⨯
Shenghui Mei,

Roles Methodology

Affiliation Department of Pharmacy, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
⨯
Hong Jin,

Roles Writing – original draft

Affiliation Institute of Disease Prevention and Control of PLA, Beijing, China
⨯
Bin Zhu,

Roles Investigation

Affiliation Department of Pharmacy, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
⨯
Yue Tian,

Roles Data curation

Affiliation Department of Pharmacy, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
⨯
Jiping Huo,

Roles Data curation

Affiliation Department of Pharmacy, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
⨯
Xu Cui,

Roles Writing – review & editing

Affiliation Neurology Research, Henry Ford Hospital, Detroit, Michigan, United States of America
⨯
Anchen Guo ,

Roles Supervision, Writing – review & editing

* E-mail: 1022zzg@sina.com (ZZ); guoanchen@hotmail.com (AG)

Affiliations Laboratory of Clinical Medicine Research, Beijing Tiantan Hospital, Capital Medical University, Beijing, China, Beijing Key Laboratory of Translational Medicine for Cerebrovascular Disease, Beijing, China, Center of Stroke, Beijing Institute for Brain Disorders, Beijing, China
⨯
Zhigang Zhao

Roles Conceptualization, Supervision

* E-mail: 1022zzg@sina.com (ZZ); guoanchen@hotmail.com (AG)

Affiliation Department of Pharmacy, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
⨯

Identification of two immortalized cell lines, ECV304 and bEnd3, for in vitro permeability studies of blood-brain barrier

Shu Yang,
Shenghui Mei,
Hong Jin,
Bin Zhu,
Yue Tian,
Jiping Huo,
Xu Cui,
Anchen Guo,
Zhigang Zhao

Published: October 23, 2017
https://doi.org/10.1371/journal.pone.0187017

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Permeability
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Blood-brain barrier
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Tight junctions
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Endothelial cells
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Blood-brain barrier permeability assay
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Cell staining
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Vascular permeability
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Immunofluorescence
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