An electroporation-free method based on Red recombineering for markerless deletion and genomic replacement in the Escherichia coli DH1 genome

• Yanlong Wei,

  Roles Data curation, Formal analysis, Investigation, Software, Validation, Writing – original draft, Writing – review & editing

  Affiliation State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China

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• Pingping Deng,

  Roles Investigation, Validation

  Affiliation Institute of Health Sciences, Anhui University, Economic and Technology Development Zone, Hefei, Anhui, China

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• Ali Mohsin,

  Roles Investigation, Validation, Writing – review & editing

  Affiliation State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China

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• Yan Yang,

  Roles Investigation, Validation

  Affiliations Luoyang Vocational & Technical College, Luoyang, Henan, China, Institute of Biotechnology, Academy of Military Medical Sciences, Beijing, China

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• Huayan Zhou,

  Roles Investigation, Validation

  Affiliation Institute of Biotechnology, Academy of Military Medical Sciences, Beijing, China

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• Meijin Guo ,

  Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Project administration, Resources, Software, Supervision, Validation, Writing – review & editing

  * E-mail: guo_mj@ecust.edu.cn (MG); fanghongqing@vip.sina.com (HF)

  Affiliation State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China

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• Hongqing Fang

  Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Software, Supervision, Validation, Writing – review & editing

  * E-mail: guo_mj@ecust.edu.cn (MG); fanghongqing@vip.sina.com (HF)

  Affiliations State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China, Institute of Health Sciences, Anhui University, Economic and Technology Development Zone, Hefei, Anhui, China, Institute of Biotechnology, Academy of Military Medical Sciences, Beijing, China

  http://orcid.org/0000-0002-2482-6830

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