A novel copro-diagnostic molecular method for qualitative detection and identification of parasitic nematodes in amphibians and reptiles

Lucas G. Huggins; Christopher J. Michaels; Sheena M. Cruickshank; Richard F. Preziosi; Kathryn J. Else

doi:10.1371/journal.pone.0185151

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Peer-reviewed

Research Article

A novel copro-diagnostic molecular method for qualitative detection and identification of parasitic nematodes in amphibians and reptiles

Lucas G. Huggins ,

Contributed equally to this work with: Lucas G. Huggins, Sheena M. Cruickshank, Richard F. Preziosi, Kathryn J. Else

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – original draft, Writing – review & editing

* E-mail: lucas-g-h@hotmail.com

Affiliation Faculty of Biology, Medicine and Health, University of Manchester, MAHSC, Manchester, United Kingdom

http://orcid.org/0000-0002-1384-2886

⨯
Christopher J. Michaels,

Roles Resources, Writing – review & editing

Affiliation Herpetology Section, ZSL London Zoo, London, United Kingdom
⨯
Sheena M. Cruickshank ,

Contributed equally to this work with: Lucas G. Huggins, Sheena M. Cruickshank, Richard F. Preziosi, Kathryn J. Else

Roles Conceptualization, Formal analysis, Investigation, Methodology, Project administration, Resources, Supervision, Validation, Writing – review & editing

Affiliation Faculty of Biology, Medicine and Health, University of Manchester, MAHSC, Manchester, United Kingdom
⨯
Richard F. Preziosi ,

Contributed equally to this work with: Lucas G. Huggins, Sheena M. Cruickshank, Richard F. Preziosi, Kathryn J. Else

Roles Conceptualization, Formal analysis, Funding acquisition, Investigation, Methodology, Resources, Supervision, Validation, Writing – review & editing

Affiliation Faculty of Science and Engineering, Manchester Metropolitan University, Manchester, United Kingdom
⨯
Kathryn J. Else

Contributed equally to this work with: Lucas G. Huggins, Sheena M. Cruickshank, Richard F. Preziosi, Kathryn J. Else

Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Validation, Visualization, Writing – review & editing

Affiliation Faculty of Biology, Medicine and Health, University of Manchester, MAHSC, Manchester, United Kingdom
⨯

A novel copro-diagnostic molecular method for qualitative detection and identification of parasitic nematodes in amphibians and reptiles

Lucas G. Huggins,
Christopher J. Michaels,
Sheena M. Cruickshank,
Richard F. Preziosi,
Kathryn J. Else

Published: September 21, 2017
https://doi.org/10.1371/journal.pone.0185151

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Nematode infections
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Parasitic diseases
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Amphibians
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Nematoda
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DNA extraction
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Signal amplification
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Polymerase chain reaction
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Reptiles
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