Improving heterologous membrane protein production in Escherichia coli by combining transcriptional tuning and codon usage algorithms

• Nico J. Claassens ,

  Contributed equally to this work with: Nico J. Claassens, Melvin F. Siliakus

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Project administration, Visualization, Writing – original draft

  * E-mail: nicoclaassens@gmail.com

  Affiliation Laboratory of Microbiology, Wageningen University and Research, Wageningen, The Netherlands

  http://orcid.org/0000-0003-1593-0377

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• Melvin F. Siliakus ,

  Contributed equally to this work with: Nico J. Claassens, Melvin F. Siliakus

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Project administration, Writing – original draft

  Current address: Department of Marine Microbiology and Biogeochemistry, NIOZ Royal Netherlands Institute for Sea Research, ′t Horntje Texel, The Netherlands

  Affiliation Laboratory of Microbiology, Wageningen University and Research, Wageningen, The Netherlands

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• Sebastiaan K. Spaans,

  Roles Methodology, Software, Writing – review & editing

  Affiliation Laboratory of Microbiology, Wageningen University and Research, Wageningen, The Netherlands

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• Sjoerd C. A. Creutzburg,

  Roles Methodology, Software, Writing – review & editing

  Affiliation Laboratory of Microbiology, Wageningen University and Research, Wageningen, The Netherlands

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• Bart Nijsse,

  Roles Software, Writing – review & editing

  Affiliation Laboratory of Systems and Synthetic Biology, Wageningen University and Research, Wageningen, The Netherlands

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• Peter J. Schaap,

  Roles Software, Supervision, Writing – review & editing

  Affiliation Laboratory of Systems and Synthetic Biology, Wageningen University and Research, Wageningen, The Netherlands

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• Tessa E. F. Quax,

  Roles Conceptualization, Writing – review & editing

  Affiliation Institut für Biologie II, Albert Ludwigs Universität Freiburg, Freiburg, Germany

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• John van der Oost

  Roles Conceptualization, Funding acquisition, Project administration, Supervision, Writing – review & editing

  Affiliation Laboratory of Microbiology, Wageningen University and Research, Wageningen, The Netherlands

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