Effect of pre-analytic variables on the reproducibility of qPCR relative telomere length measurement

Casey L. Dagnall; Belynda Hicks; Kedest Teshome; Amy A. Hutchinson; Shahinaz M. Gadalla; Payal P. Khincha; Meredith Yeager; Sharon A. Savage

doi:10.1371/journal.pone.0184098

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Open Access

Peer-reviewed

Research Article

Effect of pre-analytic variables on the reproducibility of qPCR relative telomere length measurement

Casey L. Dagnall,

Affiliations Division of Cancer Epidemiology and Genetics, National Cancer Institute (NCI), Bethesda, Maryland, United States of America, Cancer Genomics Research Laboratory, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, Frederick, Maryland, United States of America
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Belynda Hicks,

Affiliations Division of Cancer Epidemiology and Genetics, National Cancer Institute (NCI), Bethesda, Maryland, United States of America, Cancer Genomics Research Laboratory, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, Frederick, Maryland, United States of America
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Kedest Teshome,

Affiliations Division of Cancer Epidemiology and Genetics, National Cancer Institute (NCI), Bethesda, Maryland, United States of America, Cancer Genomics Research Laboratory, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, Frederick, Maryland, United States of America
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Amy A. Hutchinson,

Affiliations Division of Cancer Epidemiology and Genetics, National Cancer Institute (NCI), Bethesda, Maryland, United States of America, Cancer Genomics Research Laboratory, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, Frederick, Maryland, United States of America
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Shahinaz M. Gadalla,

Affiliation Division of Cancer Epidemiology and Genetics, National Cancer Institute (NCI), Bethesda, Maryland, United States of America
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Payal P. Khincha,

Affiliation Division of Cancer Epidemiology and Genetics, National Cancer Institute (NCI), Bethesda, Maryland, United States of America
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Meredith Yeager,

Affiliations Division of Cancer Epidemiology and Genetics, National Cancer Institute (NCI), Bethesda, Maryland, United States of America, Cancer Genomics Research Laboratory, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, Frederick, Maryland, United States of America
⨯
Sharon A. Savage

* E-mail: savagesh@mail.nih.gov

Affiliation Division of Cancer Epidemiology and Genetics, National Cancer Institute (NCI), Bethesda, Maryland, United States of America

http://orcid.org/0000-0001-6006-0740

⨯

Effect of pre-analytic variables on the reproducibility of qPCR relative telomere length measurement

Casey L. Dagnall,
Belynda Hicks,
Kedest Teshome,
Amy A. Hutchinson,
Shahinaz M. Gadalla,
Payal P. Khincha,
Meredith Yeager,
Sharon A. Savage

Published: September 8, 2017
https://doi.org/10.1371/journal.pone.0184098

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DNA extraction
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Telomere length
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Specimen storage
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Extraction techniques
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Purification techniques
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