Development of a fluorescent reporter system for monitoring ER stress in Chinese hamster ovary cells and its application for therapeutic protein production

Gargi Roy; Shu Zhang; Lina Li; Eileen Higham; Herren Wu; Marcello Marelli; Michael A. Bowen

doi:10.1371/journal.pone.0183694

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Open Access

Peer-reviewed

Research Article

Development of a fluorescent reporter system for monitoring ER stress in Chinese hamster ovary cells and its application for therapeutic protein production

Gargi Roy ,

Roles Conceptualization, Data curation, Formal analysis, Methodology, Project administration, Resources, Supervision, Visualization, Writing – original draft, Writing – review & editing

* E-mail: royg@medimmune.com (GR); m.bowen@miami.edu (MB)

Affiliation Antibody Discovery and Protein Engineering, MedImmune LLC, Gaithersburg, Maryland, United States of America

http://orcid.org/0000-0002-8391-5067

⨯
Shu Zhang,

Roles Methodology, Visualization

Current address: Biologics Development, Gilead Sciences, San Diego, California, United States of America

Affiliation Antibody Discovery and Protein Engineering, MedImmune LLC, Gaithersburg, Maryland, United States of America
⨯
Lina Li,

Roles Data curation, Investigation, Methodology

Affiliation Cell Culture and Fermentation Sciences, MedImmune LLC, Gaithersburg, Maryland, United States of America
⨯
Eileen Higham,

Roles Project administration

Current address: Process Development, Juno Therapeutics, Seattle, Washington, United States of America

Affiliation Cell Culture and Fermentation Sciences, MedImmune LLC, Gaithersburg, Maryland, United States of America
⨯
Herren Wu,

Roles Funding acquisition, Writing – review & editing

Affiliation Antibody Discovery and Protein Engineering, MedImmune LLC, Gaithersburg, Maryland, United States of America
⨯
Marcello Marelli,

Roles Writing – original draft, Writing – review & editing

Affiliation Antibody Discovery and Protein Engineering, MedImmune LLC, Gaithersburg, Maryland, United States of America
⨯
Michael A. Bowen

Roles Conceptualization, Project administration, Resources, Supervision, Writing – original draft, Writing – review & editing

* E-mail: royg@medimmune.com (GR); m.bowen@miami.edu (MB)

Current address: Analytical Development, Juno Therapeutics, Seattle, Washington, United States of America

Affiliation Antibody Discovery and Protein Engineering, MedImmune LLC, Gaithersburg, Maryland, United States of America
⨯

Development of a fluorescent reporter system for monitoring ER stress in Chinese hamster ovary cells and its application for therapeutic protein production

Gargi Roy,
Shu Zhang,
Lina Li,
Eileen Higham,
Herren Wu,
Marcello Marelli,
Michael A. Bowen

Published: August 23, 2017
https://doi.org/10.1371/journal.pone.0183694

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