Microgravity validation of a novel system for RNA isolation and multiplex quantitative real time PCR analysis of gene expression on the International Space Station

• Macarena Parra ,

  Contributed equally to this work with: Macarena Parra, Jimmy Jung

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Validation, Writing – original draft, Writing – review & editing

  Affiliation Space Biosciences Research Branch, NASA Ames Research Center, Moffett Field, California, United States of America

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• Jimmy Jung ,

  Contributed equally to this work with: Macarena Parra, Jimmy Jung

  Roles Data curation, Formal analysis, Investigation, Methodology, Writing – original draft, Writing – review & editing

  Affiliations Engineering Systems Division, NASA Ames Research Center, Moffett Field, California, United States of America, KBRWyle, Mountain View, California, United States of America

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• Travis D. Boone,

  Roles Formal analysis, Investigation, Methodology

  Affiliations Office of the Director, NASA Ames Research Center, Moffett Field, California, United States of America, Millenium Engineering & Integration Co, Mountain View, California, United States of America

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• Luan Tran,

  Roles Formal analysis, Investigation, Writing – review & editing

  Affiliations Space Biosciences Research Branch, NASA Ames Research Center, Moffett Field, California, United States of America, KBRWyle, Mountain View, California, United States of America

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• Elizabeth A. Blaber,

  Roles Conceptualization, Formal analysis, Investigation, Methodology, Writing – review & editing

  Affiliations Space Biosciences Research Branch, NASA Ames Research Center, Moffett Field, California, United States of America, Universities Space Research Association, Mountain View, California, United States of America

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• Mark Brown,

  Roles Methodology, Writing – review & editing

  Affiliation Applications Development, Claremont Biosolutions, Upland, California, United States of America

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• Matthew Chin,

  Roles Investigation, Methodology, Software, Writing – review & editing

  Affiliations Engineering Systems Division, NASA Ames Research Center, Moffett Field, California, United States of America, Millenium Engineering & Integration Co, Mountain View, California, United States of America

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• Tori Chinn,

  Roles Investigation, Methodology, Resources, Validation, Writing – review & editing

  Affiliations Engineering Systems Division, NASA Ames Research Center, Moffett Field, California, United States of America, Millenium Engineering & Integration Co, Mountain View, California, United States of America

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• Jacob Cohen,

  Roles Conceptualization, Funding acquisition, Writing – review & editing

  Affiliation Office of the Director, NASA Ames Research Center, Moffett Field, California, United States of America

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• Robert Doebler,

  Roles Methodology, Writing – review & editing

  Affiliation Applications Development, Claremont Biosolutions, Upland, California, United States of America

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• Dzung Hoang,

  Roles Investigation, Methodology, Writing – review & editing

  Affiliations Engineering Systems Division, NASA Ames Research Center, Moffett Field, California, United States of America, Millenium Engineering & Integration Co, Mountain View, California, United States of America

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• Elizabeth Hyde,

  Roles Methodology, Writing – review & editing

  Affiliations Engineering Systems Division, NASA Ames Research Center, Moffett Field, California, United States of America, Millenium Engineering & Integration Co, Mountain View, California, United States of America

  ⨯
• Matthew Lera,

  Roles Investigation, Methodology, Writing – review & editing

  Affiliations KBRWyle, Mountain View, California, United States of America, Flight Systems Implementation Branch, NASA Ames Research Center, Moffett Field, California, United States of America

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• Louie T. Luzod,

  Roles Methodology, Project administration, Writing – review & editing

  Affiliation Engineering Systems Division, NASA Ames Research Center, Moffett Field, California, United States of America

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• Mark Mallinson,

  Roles Methodology, Project administration, Writing – review & editing

  Affiliation Engineering Systems Division, NASA Ames Research Center, Moffett Field, California, United States of America

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• Oana Marcu,

  Roles Investigation, Methodology, Writing – review & editing

  Affiliations Space Biosciences Research Branch, NASA Ames Research Center, Moffett Field, California, United States of America, KBRWyle, Mountain View, California, United States of America

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• Youssef Mohamedaly,

  Roles Investigation, Methodology, Software, Writing – review & editing

  Affiliations Engineering Systems Division, NASA Ames Research Center, Moffett Field, California, United States of America, Millenium Engineering & Integration Co, Mountain View, California, United States of America

  ⨯
• Antonio J. Ricco,

  Roles Conceptualization, Writing – review & editing

  Affiliations Mission Design Division, NASA Ames Research Center, Moffett Field, California, United States of America, Stanford University, Palo Alto, California, United States of America

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• Kathleen Rubins,

  Roles Investigation, Writing – review & editing

  Affiliation NASA Astronaut Corps, NASA Johnson Space Center, Houston, Texas, United States of America

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• Gregory D. Sgarlato,

  Roles Investigation, Writing – review & editing

  Affiliations Engineering Systems Division, NASA Ames Research Center, Moffett Field, California, United States of America, KBRWyle, Mountain View, California, United States of America

  ⨯
• Rafael O. Talavera,

  Roles Methodology, Writing – review & editing

  Affiliations Engineering Systems Division, NASA Ames Research Center, Moffett Field, California, United States of America, Millenium Engineering & Integration Co, Mountain View, California, United States of America

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• Peter Tong,

  Roles Conceptualization, Investigation, Methodology, Writing – review & editing

  Affiliations Engineering Systems Division, NASA Ames Research Center, Moffett Field, California, United States of America, Millenium Engineering & Integration Co, Mountain View, California, United States of America

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• Eddie Uribe,

  Roles Investigation, Methodology, Writing – review & editing

  Affiliation Universities Space Research Association, Mountain View, California, United States of America

  ⨯
• Jeffrey Williams,

  Roles Investigation, Writing – review & editing

  Affiliation NASA Astronaut Corps, NASA Johnson Space Center, Houston, Texas, United States of America

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• Diana Wu,

  Roles Investigation, Methodology, Writing – review & editing

  Affiliations KBRWyle, Mountain View, California, United States of America, Mission Design Division, NASA Ames Research Center, Moffett Field, California, United States of America

  ⨯
• Rukhsana Yousuf,

  Roles Investigation, Methodology, Writing – review & editing

  Affiliations Space Biosciences Research Branch, NASA Ames Research Center, Moffett Field, California, United States of America, KBRWyle, Mountain View, California, United States of America

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• Charles S. Richey,

  Roles Funding acquisition, Project administration, Supervision, Writing – review & editing

  Affiliation Universities Space Research Association, Mountain View, California, United States of America

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• Julie Schonfeld,

  Roles Funding acquisition, Project administration, Supervision, Writing – review & editing

  Affiliation Engineering Systems Division, NASA Ames Research Center, Moffett Field, California, United States of America

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•  [ ... ],
• Eduardo A. C. Almeida

  Roles Conceptualization, Formal analysis, Investigation, Methodology, Resources, Supervision, Validation, Writing – original draft, Writing – review & editing

  * E-mail: e.almeida@nasa.gov

  Affiliation Space Biosciences Research Branch, NASA Ames Research Center, Moffett Field, California, United States of America

  http://orcid.org/0000-0002-3420-6651

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