Synthetic mRNA is a more reliable tool for the delivery of DNA-targeting proteins into the cell nucleus than fusion with a protein transduction domain

Ivan Leontovyc; David Habart; Sarka Loukotova; Lucie Kosinova; Jan Kriz; Frantisek Saudek; Tomas Koblas

doi:10.1371/journal.pone.0182497

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Open Access

Peer-reviewed

Research Article

Synthetic mRNA is a more reliable tool for the delivery of DNA-targeting proteins into the cell nucleus than fusion with a protein transduction domain

Ivan Leontovyc ,

Contributed equally to this work with: Ivan Leontovyc, David Habart, Sarka Loukotova, Lucie Kosinova, Tomas Koblas

Roles Formal analysis, Investigation, Writing – original draft

* E-mail: ivan.leontovyc@ikem.cz

Affiliation Department of Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic

http://orcid.org/0000-0003-1610-7796

⨯
David Habart ,

Contributed equally to this work with: Ivan Leontovyc, David Habart, Sarka Loukotova, Lucie Kosinova, Tomas Koblas

Roles Writing – review & editing

Affiliation Department of Diabetes, Institute for Clinical and Experimental Medicine, Prague, Czech Republic
⨯
Sarka Loukotova ,

Contributed equally to this work with: Ivan Leontovyc, David Habart, Sarka Loukotova, Lucie Kosinova, Tomas Koblas

Roles Formal analysis, Investigation

Affiliation Department of Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic
⨯
Lucie Kosinova ,

Contributed equally to this work with: Ivan Leontovyc, David Habart, Sarka Loukotova, Lucie Kosinova, Tomas Koblas

Roles Formal analysis

Affiliation Department of Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic
⨯
Jan Kriz ,

Roles Resources

¶‡ These authors also contributed equally to this work.

Affiliation Department of Diabetes, Institute for Clinical and Experimental Medicine, Prague, Czech Republic
⨯
Frantisek Saudek ,

Roles Resources

¶‡ These authors also contributed equally to this work.

Affiliation Department of Diabetes, Institute for Clinical and Experimental Medicine, Prague, Czech Republic
⨯
Tomas Koblas

Contributed equally to this work with: Ivan Leontovyc, David Habart, Sarka Loukotova, Lucie Kosinova, Tomas Koblas

Roles Conceptualization, Methodology, Resources, Supervision

Affiliation Department of Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic
⨯

Synthetic mRNA is a more reliable tool for the delivery of DNA-targeting proteins into the cell nucleus than fusion with a protein transduction domain

Ivan Leontovyc,
David Habart,
Sarka Loukotova,
Lucie Kosinova,
Jan Kriz,
Frantisek Saudek,
Tomas Koblas

Published: August 14, 2017
https://doi.org/10.1371/journal.pone.0182497

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Recombinant proteins
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Green fluorescent protein
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Messenger RNA
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Transcription factors
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