LC-MS/MS versus TLC plus GC methods: Consistency of glycerolipid and fatty acid profiles in microalgae and higher plant cells and effect of a nitrogen starvation

Juliette Jouhet; Josselin Lupette; Olivier Clerc; Leonardo Magneschi; Mariette Bedhomme; Séverine Collin; Sylvaine Roy; Eric Maréchal; Fabrice Rébeillé

doi:10.1371/journal.pone.0182423

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Peer-reviewed

Research Article

LC-MS/MS versus TLC plus GC methods: Consistency of glycerolipid and fatty acid profiles in microalgae and higher plant cells and effect of a nitrogen starvation

Juliette Jouhet,

Roles Conceptualization, Data curation, Investigation, Methodology, Supervision, Writing – original draft

Affiliation Laboratoire de Physiologie Cellulaire Végétale, Unité mixte de recherche 5168 CNRS - CEA - INRA - Université Grenoble Alpes, Bioscience and Biotechnologies Institute of Grenoble, CEA Grenoble, Grenoble, France
⨯
Josselin Lupette,

Roles Data curation, Investigation

Affiliation Laboratoire de Physiologie Cellulaire Végétale, Unité mixte de recherche 5168 CNRS - CEA - INRA - Université Grenoble Alpes, Bioscience and Biotechnologies Institute of Grenoble, CEA Grenoble, Grenoble, France
⨯
Olivier Clerc,

Roles Data curation, Software

Affiliation Laboratoire de Physiologie Cellulaire Végétale, Unité mixte de recherche 5168 CNRS - CEA - INRA - Université Grenoble Alpes, Bioscience and Biotechnologies Institute of Grenoble, CEA Grenoble, Grenoble, France
⨯
Leonardo Magneschi,

Roles Investigation, Methodology

Affiliation Laboratoire de Physiologie Cellulaire Végétale, Unité mixte de recherche 5168 CNRS - CEA - INRA - Université Grenoble Alpes, Bioscience and Biotechnologies Institute of Grenoble, CEA Grenoble, Grenoble, France
⨯
Mariette Bedhomme,

Roles Investigation, Methodology

Affiliation Laboratoire de Physiologie Cellulaire Végétale, Unité mixte de recherche 5168 CNRS - CEA - INRA - Université Grenoble Alpes, Bioscience and Biotechnologies Institute of Grenoble, CEA Grenoble, Grenoble, France
⨯
Séverine Collin,

Roles Investigation, Methodology

Affiliation Laboratoire de Physiologie Cellulaire Végétale, Unité mixte de recherche 5168 CNRS - CEA - INRA - Université Grenoble Alpes, Bioscience and Biotechnologies Institute of Grenoble, CEA Grenoble, Grenoble, France
⨯
Sylvaine Roy,

Roles Methodology, Software, Validation

Affiliation Laboratoire de Physiologie Cellulaire Végétale, Unité mixte de recherche 5168 CNRS - CEA - INRA - Université Grenoble Alpes, Bioscience and Biotechnologies Institute of Grenoble, CEA Grenoble, Grenoble, France
⨯
Eric Maréchal,

Roles Supervision, Validation

Affiliation Laboratoire de Physiologie Cellulaire Végétale, Unité mixte de recherche 5168 CNRS - CEA - INRA - Université Grenoble Alpes, Bioscience and Biotechnologies Institute of Grenoble, CEA Grenoble, Grenoble, France
⨯
Fabrice Rébeillé

Roles Conceptualization, Data curation, Methodology, Validation, Writing – original draft, Writing – review & editing

* E-mail: fabrice.rebeille@cea.fr

Affiliation Laboratoire de Physiologie Cellulaire Végétale, Unité mixte de recherche 5168 CNRS - CEA - INRA - Université Grenoble Alpes, Bioscience and Biotechnologies Institute of Grenoble, CEA Grenoble, Grenoble, France

http://orcid.org/0000-0001-6132-8542

⨯

LC-MS/MS versus TLC plus GC methods: Consistency of glycerolipid and fatty acid profiles in microalgae and higher plant cells and effect of a nitrogen starvation

Juliette Jouhet,
Josselin Lupette,
Olivier Clerc,
Leonardo Magneschi,
Mariette Bedhomme,
Séverine Collin,
Sylvaine Roy,
Eric Maréchal,
Fabrice Rébeillé

Published: August 3, 2017
https://doi.org/10.1371/journal.pone.0182423

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Lipids
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Thin-layer chromatography
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Lipid analysis
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Arabidopsis thaliana
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Fatty acids
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Glycerol
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