Metabolic engineering of CHO cells for the development of a robust protein production platform

Sanjeev Kumar Gupta; Santosh K. Srivastava; Ankit Sharma; Vaibhav H. H. Nalage; Darshita Salvi; Hiralal Kushwaha; Nikhil B. Chitnis; Pratyoosh Shukla

doi:10.1371/journal.pone.0181455

Loading metrics

Open Access

Peer-reviewed

Research Article

Metabolic engineering of CHO cells for the development of a robust protein production platform

Sanjeev Kumar Gupta ,

Contributed equally to this work with: Sanjeev Kumar Gupta, Santosh K. Srivastava

Roles Conceptualization, Resources, Validation, Visualization, Writing – original draft

Affiliations Advanced Biotech Lab, Ipca Laboratories Ltd., Plot#125, Kandivli Industrial Estate, Kandivli (west), Mumbai, Maharashtra, India, Enzyme Technology and Protein Bioinformatics Laboratory, Department of Microbiology, Maharshi Dayanand University, Rohtak, Haryana-India
⨯
Santosh K. Srivastava ,

Contributed equally to this work with: Sanjeev Kumar Gupta, Santosh K. Srivastava

Roles Conceptualization, Data curation, Validation, Visualization, Writing – original draft

Affiliation Advanced Biotech Lab, Ipca Laboratories Ltd., Plot#125, Kandivli Industrial Estate, Kandivli (west), Mumbai, Maharashtra, India
⨯
Ankit Sharma,

Roles Data curation

Affiliation Advanced Biotech Lab, Ipca Laboratories Ltd., Plot#125, Kandivli Industrial Estate, Kandivli (west), Mumbai, Maharashtra, India

http://orcid.org/0000-0002-9955-3320

⨯
Vaibhav H. H. Nalage,

Roles Methodology

Affiliation Advanced Biotech Lab, Ipca Laboratories Ltd., Plot#125, Kandivli Industrial Estate, Kandivli (west), Mumbai, Maharashtra, India
⨯
Darshita Salvi,

Roles Methodology

Affiliation Advanced Biotech Lab, Ipca Laboratories Ltd., Plot#125, Kandivli Industrial Estate, Kandivli (west), Mumbai, Maharashtra, India

http://orcid.org/0000-0001-6825-2990

⨯
Hiralal Kushwaha,

Roles Formal analysis

Affiliation Advanced Biotech Lab, Ipca Laboratories Ltd., Plot#125, Kandivli Industrial Estate, Kandivli (west), Mumbai, Maharashtra, India
⨯
Nikhil B. Chitnis,

Roles Formal analysis

Affiliation Advanced Biotech Lab, Ipca Laboratories Ltd., Plot#125, Kandivli Industrial Estate, Kandivli (west), Mumbai, Maharashtra, India

http://orcid.org/0000-0001-8132-8400

⨯
Pratyoosh Shukla

Roles Conceptualization, Supervision, Writing – review & editing

* E-mail: pratyoosh.shukla@gmail.com

Affiliation Enzyme Technology and Protein Bioinformatics Laboratory, Department of Microbiology, Maharshi Dayanand University, Rohtak, Haryana-India
⨯

Metabolic engineering of CHO cells for the development of a robust protein production platform

Sanjeev Kumar Gupta,
Santosh K. Srivastava,
Ankit Sharma,
Vaibhav H. H. Nalage,
Darshita Salvi,
Hiralal Kushwaha,
Nikhil B. Chitnis,
Pratyoosh Shukla

Published: August 1, 2017
https://doi.org/10.1371/journal.pone.0181455

Reader Comments (0)

Post a new comment on this article

Subject Areas
?

For more information about PLOS Subject Areas, click here.
We want your feedback. Do these Subject Areas make sense for this article? Click the target next to the incorrect Subject Area and let us know. Thanks for your help!

CHO cells
Is the Subject Area "CHO cells" applicable to this article?

Thanks for your feedback.
Cloning
Is the Subject Area "Cloning" applicable to this article?

Thanks for your feedback.
Gene pool
Is the Subject Area "Gene pool" applicable to this article?

Thanks for your feedback.
Cell metabolism
Is the Subject Area "Cell metabolism" applicable to this article?

Thanks for your feedback.
Pyruvate
Is the Subject Area "Pyruvate" applicable to this article?

Thanks for your feedback.
Glucose metabolism
Is the Subject Area "Glucose metabolism" applicable to this article?

Thanks for your feedback.
Gene cloning
Is the Subject Area "Gene cloning" applicable to this article?

Thanks for your feedback.
Cell cultures
Is the Subject Area "Cell cultures" applicable to this article?

Thanks for your feedback.