Increased genetic diversity and prevalence of co-infection with Trypanosoma spp. in koalas (Phascolarctos cinereus) and their ticks identified using next-generation sequencing (NGS)

Amanda D. Barbosa; Alexander W. Gofton; Andrea Paparini; Annachiara Codello; Telleasha Greay; Amber Gillett; Kristin Warren; Peter Irwin; Una Ryan

doi:10.1371/journal.pone.0181279

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Open Access

Peer-reviewed

Research Article

Increased genetic diversity and prevalence of co-infection with Trypanosoma spp. in koalas (Phascolarctos cinereus) and their ticks identified using next-generation sequencing (NGS)

Amanda D. Barbosa ,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – original draft, Writing – review & editing

* E-mail: A.DuarteBarbosa@murdoch.edu.au

Affiliations School of Veterinary and Life Sciences, Murdoch University, Murdoch, Perth, Western Australia, CAPES Foundation, Ministry of Education of Brazil, Brasília, Distrito Federal, Brazil

http://orcid.org/0000-0003-3289-1445

⨯
Alexander W. Gofton,

Roles Data curation, Investigation, Methodology, Validation, Writing – review & editing

Affiliation School of Veterinary and Life Sciences, Murdoch University, Murdoch, Perth, Western Australia
⨯
Andrea Paparini,

Roles Data curation, Formal analysis, Methodology, Validation, Writing – review & editing

Affiliation School of Veterinary and Life Sciences, Murdoch University, Murdoch, Perth, Western Australia
⨯
Annachiara Codello,

Roles Investigation, Writing – review & editing

Affiliation School of Veterinary and Life Sciences, Murdoch University, Murdoch, Perth, Western Australia
⨯
Telleasha Greay,

Roles Investigation, Writing – review & editing

Affiliation School of Veterinary and Life Sciences, Murdoch University, Murdoch, Perth, Western Australia
⨯
Amber Gillett,

Roles Resources, Writing – review & editing

Affiliations Australia Zoo Wildlife Hospital, Beerwah, Queensland, Australia, School of Veterinary Science, University of Queensland, Saint Lucia, Australia
⨯
Kristin Warren,

Roles Resources, Supervision, Writing – review & editing

Affiliation School of Veterinary and Life Sciences, Murdoch University, Murdoch, Perth, Western Australia
⨯
Peter Irwin,

Roles Resources, Supervision, Writing – review & editing

Affiliation School of Veterinary and Life Sciences, Murdoch University, Murdoch, Perth, Western Australia
⨯
Una Ryan

Roles Conceptualization, Project administration, Resources, Supervision, Writing – review & editing

Affiliation School of Veterinary and Life Sciences, Murdoch University, Murdoch, Perth, Western Australia
⨯

Increased genetic diversity and prevalence of co-infection with Trypanosoma spp. in koalas (Phascolarctos cinereus) and their ticks identified using next-generation sequencing (NGS)

Amanda D. Barbosa,
Alexander W. Gofton,
Andrea Paparini,
Annachiara Codello,
Telleasha Greay,
Amber Gillett,
Kristin Warren,
Peter Irwin,
Una Ryan

Published: July 13, 2017
https://doi.org/10.1371/journal.pone.0181279

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Trypanosoma
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Next-generation sequencing
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Dideoxy DNA sequencing
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Polymerase chain reaction
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Phylogenetic analysis
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Ixodes
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Chlamydia infection
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