Amplification of overlapping DNA amplicons in a single-tube multiplex PCR for targeted next-generation sequencing of BRCA1 and BRCA2

Open Access

Peer-reviewed

Research Article

Desiree Schenk,

Affiliation Department of Research and Development, Pillar Biosciences, Natick, Massachusetts, United States of America
⨯
Gang Song,

Affiliation Department of Research and Development, Pillar Biosciences, Natick, Massachusetts, United States of America
⨯
Yue Ke,

Affiliation Department of Research and Development, Pillar Biosciences, Natick, Massachusetts, United States of America
⨯
Zhaohui Wang

* E-mail: wangzh@pillar-biosciences.com

Affiliation Department of Research and Development, Pillar Biosciences, Natick, Massachusetts, United States of America
⨯

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Polymerase chain reaction
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DNA libraries
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Next-generation sequencing
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DNA annealing
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Gel electrophoresis
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Genetic annealing
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Introns
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Oligonucleotides
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