RNAi-based reverse genetics in the chelicerate model Tetranychus urticae: A comparative analysis of five methods for gene silencing

• Takeshi Suzuki ,

  Contributed equally to this work with: Takeshi Suzuki, Maria Andreia Nunes, María Urizarna España

  Current address: Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo, Japan

  Affiliation Department of Biology, The University of Western Ontario, London, Ontario, Canada

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• Maria Andreia Nunes ,

  Contributed equally to this work with: Takeshi Suzuki, Maria Andreia Nunes, María Urizarna España

  Current address: Centro de Citricultura Sylvio Moreira, Instituto Agronômico de Campinas, Cordeirópolis, São Paulo, Brazil

  Affiliation Department of Biology, The University of Western Ontario, London, Ontario, Canada

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• María Urizarna España ,

  Contributed equally to this work with: Takeshi Suzuki, Maria Andreia Nunes, María Urizarna España

  Affiliation Department of Biology, The University of Western Ontario, London, Ontario, Canada

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• Hooman Hosseinzadeh Namin,

  Affiliation Department of Biology, The University of Western Ontario, London, Ontario, Canada

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• Pengyu Jin,

  Affiliation Department of Biology, The University of Western Ontario, London, Ontario, Canada

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• Nicolas Bensoussan,

  Affiliation Department of Biology, The University of Western Ontario, London, Ontario, Canada

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• Vladimir Zhurov,

  Affiliation Department of Biology, The University of Western Ontario, London, Ontario, Canada

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• Tawhid Rahman,

  Current address: Saskatoon Research Centre, Agriculture and Agri-Food Canada, Saskatoon, SK, Canada

  Affiliation Department of Biology, The University of Western Ontario, London, Ontario, Canada

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• Rebecca De Clercq,

  Affiliation Department of Plant Systems Biology, VIB, Ghent, Belgium

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• Pierre Hilson,

  Affiliations Department of Plant Systems Biology, VIB, Ghent, Belgium, Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium, Institut Jean-Pierre Bourgin, UMR1318 INRA-AgroParisTech, Saclay Plant Science, Versailles, France

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• Vojislava Grbic ,

  * E-mail: mgrbic@uwo.ca (MG); vgrbic@uwo.ca (VG)

  Affiliation Department of Biology, The University of Western Ontario, London, Ontario, Canada

  http://orcid.org/0000-0001-6570-8796

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• Miodrag Grbic

  * E-mail: mgrbic@uwo.ca (MG); vgrbic@uwo.ca (VG)

  Affiliations Department of Biology, The University of Western Ontario, London, Ontario, Canada, University of La Rioja, Logrono, Spain

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