Rapid detection and strain typing of Chlamydia trachomatis using a highly multiplexed microfluidic PCR assay

Rosemary S. Turingan; Ludmila Kaplun; Greice Krautz-Peterson; Sarah Norsworthy; Anna Zolotova; Sandeep J. Joseph; Timothy D. Read; Deborah Dean; Eugene Tan; Richard F. Selden

doi:10.1371/journal.pone.0178653

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Open Access

Peer-reviewed

Research Article

Rapid detection and strain typing of Chlamydia trachomatis using a highly multiplexed microfluidic PCR assay

Rosemary S. Turingan,

Affiliation NetBio, Waltham, Massachusetts, United States of America
⨯
Ludmila Kaplun,

Affiliation NetBio, Waltham, Massachusetts, United States of America
⨯
Greice Krautz-Peterson,

Affiliation NetBio, Waltham, Massachusetts, United States of America
⨯
Sarah Norsworthy,

Affiliation NetBio, Waltham, Massachusetts, United States of America
⨯
Anna Zolotova,

Affiliation NetBio, Waltham, Massachusetts, United States of America
⨯
Sandeep J. Joseph,

Affiliation Department of Medicine, Division of Infectious Diseases and Department of Human Genetics, Emory University School of Medicine, Atlanta, Georgia, United States of America
⨯
Timothy D. Read,

Affiliation Department of Medicine, Division of Infectious Diseases and Department of Human Genetics, Emory University School of Medicine, Atlanta, Georgia, United States of America
⨯
Deborah Dean,

Affiliations Center for Immunobiology and Vaccine Development, Children’s Hospital Oakland Research Institute, Oakland, California, United States of America, University of California at San Francisco School of Medicine, San Francisco, California, United States of America, University of California at Berkeley and University of California at San Francisco Joint Graduate Program in Bioengineering, Berkeley, California, United States of America
⨯
Eugene Tan,

Affiliation NetBio, Waltham, Massachusetts, United States of America
⨯
Richard F. Selden

* E-mail: richard.selden@netbio.com

Affiliation NetBio, Waltham, Massachusetts, United States of America

http://orcid.org/0000-0002-0741-0913

⨯

Rapid detection and strain typing of Chlamydia trachomatis using a highly multiplexed microfluidic PCR assay

Rosemary S. Turingan,
Ludmila Kaplun,
Greice Krautz-Peterson,
Sarah Norsworthy,
Anna Zolotova,
Sandeep J. Joseph,
Timothy D. Read,
Deborah Dean,
Eugene Tan,
Richard F. Selden

Published: May 31, 2017
https://doi.org/10.1371/journal.pone.0178653

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Polymerase chain reaction
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Sequence alignment
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DNA purification
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