SensiScreen® KRAS exon 2-sensitive simplex and multiplex real-time PCR-based assays for detection of KRAS exon 2 mutations

Alice Riva; Michael BØrgesen; Mariann Guldmann-Christensen; Majbritt Hauge Kyneb; Kirsten Voogd; Christina Andersen; Samantha Epistolio; Elisabetta Merlo; Tine Yding Wolff; Stephen Hamilton-Dutoit; Jan Lorenzen; Ulf Bech Christensen; Milo Frattini

doi:10.1371/journal.pone.0178027

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Open Access

Peer-reviewed

Research Article

SensiScreen^® KRAS exon 2-sensitive simplex and multiplex real-time PCR-based assays for detection of KRAS exon 2 mutations

Alice Riva ,

Contributed equally to this work with: Alice Riva, Michael BØrgesen

Affiliation Laboratory of Molecular Pathology, Institute of Pathology, Locarno, Switzerland
⨯
Michael BØrgesen ,

Contributed equally to this work with: Alice Riva, Michael BØrgesen

Affiliation PentaBase Aps, Odense, Denmark
⨯
Mariann Guldmann-Christensen,

Affiliation Laboratory of Research and Development, Department of Pathology, Aarhus University Hospital, Aarhus, Denmark
⨯
Majbritt Hauge Kyneb,

Affiliation Life Science Division, Danish Technological Institute, Aarhus, Denmark
⨯
Kirsten Voogd,

Current address: University of Southern Denmark, Odense, Denmark

Affiliation Laboratory of Research and Development, Department of Pathology, Aarhus University Hospital, Aarhus, Denmark
⨯
Christina Andersen,

Affiliation PentaBase Aps, Odense, Denmark
⨯
Samantha Epistolio,

Affiliation Laboratory of Molecular Pathology, Institute of Pathology, Locarno, Switzerland
⨯
Elisabetta Merlo,

Affiliation Laboratory of Molecular Pathology, Institute of Pathology, Locarno, Switzerland
⨯
Tine Yding Wolff,

Current address: Aarhus School of Engineering, Aarhus, Denmark

Affiliation Life Science Division, Danish Technological Institute, Aarhus, Denmark
⨯
Stephen Hamilton-Dutoit,

Affiliation Laboratory of Research and Development, Department of Pathology, Aarhus University Hospital, Aarhus, Denmark
⨯
Jan Lorenzen,

Affiliation Life Science Division, Danish Technological Institute, Aarhus, Denmark
⨯
Ulf Bech Christensen ,

* E-mail: ubc@PentaBase.com (UBC); milo.frattini@ti.ch (MF)

¶‡ These authors are Joint Senior Authors.

Affiliation PentaBase Aps, Odense, Denmark
⨯
Milo Frattini

* E-mail: ubc@PentaBase.com (UBC); milo.frattini@ti.ch (MF)

¶‡ These authors are Joint Senior Authors.

Affiliation Laboratory of Molecular Pathology, Institute of Pathology, Locarno, Switzerland

http://orcid.org/0000-0003-2334-3412

⨯

SensiScreen^® KRAS exon 2-sensitive simplex and multiplex real-time PCR-based assays for detection of KRAS exon 2 mutations

Alice Riva,
Michael BØrgesen,
Mariann Guldmann-Christensen,
Majbritt Hauge Kyneb,
Kirsten Voogd,
Christina Andersen,
Samantha Epistolio,
Elisabetta Merlo,
Tine Yding Wolff,
Stephen Hamilton-Dutoit

Published: June 21, 2017
https://doi.org/10.1371/journal.pone.0178027

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