Chemical and structural characterization of a model Post-Termination Complex (PoTC) for the ribosome recycling reaction: Evidence for the release of the mRNA by RRF and EF-G

Nobuhiro Iwakura; Takeshi Yokoyama; Fabio Quaglia; Kaoru Mitsuoka; Kazuhiro Mio; Hideki Shigematsu; Mikako Shirouzu; Akira Kaji; Hideko Kaji

doi:10.1371/journal.pone.0177972

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Peer-reviewed

Research Article

Chemical and structural characterization of a model Post-Termination Complex (PoTC) for the ribosome recycling reaction: Evidence for the release of the mRNA by RRF and EF-G

Nobuhiro Iwakura,

Affiliations Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Jefferson Medical College, Philadelphia, Pennsylvania, United States of America, Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, United States of America
⨯
Takeshi Yokoyama,

Affiliation Division of Structural and Synthetic Biology, RIKEN Center for Life Science Technologies, Yokohama, Japan
⨯
Fabio Quaglia,

Affiliations Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Jefferson Medical College, Philadelphia, Pennsylvania, United States of America, Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, United States of America, University of Camerino, School of Biosciences and Veterinary Medicine, Camerino, Italy
⨯
Kaoru Mitsuoka,

Affiliation Research Center for Ultra-High Voltage Electron Microscopy, Osaka University, Osaka, Japan
⨯
Kazuhiro Mio,

Affiliation Molecular Profiling Research Center for Drug Discovery and OPERANDO Open Innovation Laboratory, National Institute of Advanced Industrial Science and Technology, Tokyo, Japan
⨯
Hideki Shigematsu,

Affiliation Division of Structural and Synthetic Biology, RIKEN Center for Life Science Technologies, Yokohama, Japan
⨯
Mikako Shirouzu,

Affiliation Division of Structural and Synthetic Biology, RIKEN Center for Life Science Technologies, Yokohama, Japan
⨯
Akira Kaji ,

* E-mail: hideko.kaji@jefferson.edu (HK); kaji@mail.med.upenn.edu (AK)

Affiliation Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, United States of America
⨯
Hideko Kaji

* E-mail: hideko.kaji@jefferson.edu (HK); kaji@mail.med.upenn.edu (AK)

Affiliation Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Jefferson Medical College, Philadelphia, Pennsylvania, United States of America
⨯

Chemical and structural characterization of a model Post-Termination Complex (PoTC) for the ribosome recycling reaction: Evidence for the release of the mRNA by RRF and EF-G

Nobuhiro Iwakura,
Takeshi Yokoyama,
Fabio Quaglia,
Kaoru Mitsuoka,
Kazuhiro Mio,
Hideki Shigematsu,
Mikako Shirouzu,
Akira Kaji,
Hideko Kaji

Published: May 24, 2017
https://doi.org/10.1371/journal.pone.0177972

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Transfer RNA
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Ribosomes
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Polyribosomes
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Messenger RNA
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Ribosomal RNA
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Sedimentation
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RNA structure
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