Development of an R4 dual-site (R4DS) gateway cloning system enabling the efficient simultaneous cloning of two desired sets of promoters and open reading frames in a binary vector for plant research

• Mostafa Aboulela,

  Affiliations Department of Molecular and Functional Genomics, Interdisciplinary Center for Science Research, Organization for Research, Shimane University, Matsue, Japan, Bioresources Science, The United Graduate School of Agricultural Sciences, Tottori University, Tottori, Japan, Department of Botany and Microbiology, Faculty of Science, Assiut University, Assiut, Egypt

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• Yuji Tanaka,

  Affiliation Department of Molecular and Functional Genomics, Interdisciplinary Center for Science Research, Organization for Research, Shimane University, Matsue, Japan

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• Kohji Nishimura,

  Affiliations Department of Molecular and Functional Genomics, Interdisciplinary Center for Science Research, Organization for Research, Shimane University, Matsue, Japan, Bioresources Science, The United Graduate School of Agricultural Sciences, Tottori University, Tottori, Japan

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• Shoji Mano,

  Affiliations Department of Evolutionary Biology and Biodiversity, National Institute for Basic Biology, Okazaki, Japan, Department of Basic Biology, School of Life Science, SOKENDAI (The Graduate University for Advanced Studies), Okazaki, Japan

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• Mikio Nishimura,

  Current address: Research Enhancement Strategy Office, National Institute for Basic Biology, Okazaki, Japan

  Affiliation Department of Cell Biology, National Institute for Basic Biology, Okazaki, Japan

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• Sumie Ishiguro,

  Affiliation Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan

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• Tetsuya Kimura,

  Affiliation Department of Life Sciences, Graduate School of Bioresources, Mie University, Tsu, Japan

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• Tsuyoshi Nakagawa

  * E-mail: tnakagaw@life.shimane-u.ac.jp

  Affiliations Department of Molecular and Functional Genomics, Interdisciplinary Center for Science Research, Organization for Research, Shimane University, Matsue, Japan, Bioresources Science, The United Graduate School of Agricultural Sciences, Tottori University, Tottori, Japan

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