Efficient method for site-directed mutagenesis in large plasmids without subcloning

• Louay K. Hallak ,

  * E-mail: lhallak@firstbiotech.com

  Affiliations Center for Vaccines and Immunity, The Research Institute at Nationwide Children’s Hospital, Columbus, Ohio, United States of America, First Biotech Inc., Athens, Ohio, United States of America

  http://orcid.org/0000-0001-5169-3626

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• Kelly Berger,

  Current address: Genetic Counseling Program, College of Medicine, University of Cincinnati and Division of Human Genetics, Cincinnati Children’s Hospital Medical Center, Cincinnati, Ohio, United States of America

  Affiliations Center for Vaccines and Immunity, The Research Institute at Nationwide Children’s Hospital, Columbus, Ohio, United States of America, Heidelberg College, Tiffin, Ohio, United States of America

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• Rita Kaspar,

  Affiliations Center for Gene Therapy, The Research Institute at Nationwide Children’s Hospital, Columbus, Ohio, United States of America, Department of Pediatrics, The Ohio State University College of Medicine, Columbus, Ohio, United States of America

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• Anna R. Kwilas,

  Current address: Gene Therapy Branch, FDA/CBER/OCTGT/DCGT, Silver Spring, Maryland, United States of America

  Affiliations Center for Vaccines and Immunity, The Research Institute at Nationwide Children’s Hospital, Columbus, Ohio, United States of America, Integrated Biomedical Sciences Graduate Program, The Ohio State University College of Medicine, Columbus, Ohio, United States of America

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• Federica Montanaro,

  Current address: The Institute of Child Health, University College London, Dubowitz Neuromuscular Centre, London, United Kingdom

  Affiliation Center for Gene Therapy, The Research Institute at Nationwide Children’s Hospital, Columbus, Ohio, United States of America

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• Mark E. Peeples

  Affiliations Center for Vaccines and Immunity, The Research Institute at Nationwide Children’s Hospital, Columbus, Ohio, United States of America, Department of Pediatrics, The Ohio State University College of Medicine, Columbus, Ohio, United States of America

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