Plant cell wall glycosyltransferases: High-throughput recombinant expression screening and general requirements for these challenging enzymes

Ditte Hededam Welner; David Shin; Giovani P. Tomaleri; Andy M. DeGiovanni; Alex Yi-Lin Tsai; Huu M. Tran; Sara Fasmer Hansen; Derek T. Green; Henrik V. Scheller; Paul D. Adams

doi:10.1371/journal.pone.0177591

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Open Access

Peer-reviewed

Research Article

Plant cell wall glycosyltransferases: High-throughput recombinant expression screening and general requirements for these challenging enzymes

Ditte Hededam Welner ,

* E-mail: diwel@dtu.dk

Current address: Department of Biotechnology and Biomedicine, Technical University of Denmark, Lyngby, Denmark

Affiliations Joint BioEnergy Institute, Emeryville, California, United States of America, Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, United States of America

http://orcid.org/0000-0001-9297-4133

⨯
David Shin,

Affiliations Joint BioEnergy Institute, Emeryville, California, United States of America, Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, United States of America
⨯
Giovani P. Tomaleri,

Affiliations Joint BioEnergy Institute, Emeryville, California, United States of America, Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, United States of America
⨯
Andy M. DeGiovanni,

Affiliations Joint BioEnergy Institute, Emeryville, California, United States of America, Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, United States of America
⨯
Alex Yi-Lin Tsai,

Affiliations Joint BioEnergy Institute, Emeryville, California, United States of America, Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, California, United States of America
⨯
Huu M. Tran,

Affiliations Joint BioEnergy Institute, Emeryville, California, United States of America, Biological and Engineering Sciences Center, Sandia National Laboratories, Livermore, California, United States of America
⨯
Sara Fasmer Hansen,

Affiliations Joint BioEnergy Institute, Emeryville, California, United States of America, Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, California, United States of America
⨯
Derek T. Green,
Henrik V. Scheller,

Affiliations Joint BioEnergy Institute, Emeryville, California, United States of America, Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, California, United States of America
⨯
Paul D. Adams

Affiliations Joint BioEnergy Institute, Emeryville, California, United States of America, Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, United States of America
⨯

Plant cell wall glycosyltransferases: High-throughput recombinant expression screening and general requirements for these challenging enzymes

Ditte Hededam Welner,
David Shin,
Giovani P. Tomaleri,
Andy M. DeGiovanni,
Alex Yi-Lin Tsai,
Huu M. Tran,
Sara Fasmer Hansen,
Derek T. Green,
Henrik V. Scheller,
Paul D. Adams

Published: June 9, 2017
https://doi.org/10.1371/journal.pone.0177591

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