Expression in Sf9 insect cells, purification and functional reconstitution of the human proton-coupled folate transporter (PCFT, SLC46A1)

Swapneeta S. Date; Mariana C. Fiori; Guillermo A. Altenberg; Michaela Jansen

doi:10.1371/journal.pone.0177572

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Research Article

Expression in Sf9 insect cells, purification and functional reconstitution of the human proton-coupled folate transporter (PCFT, SLC46A1)

Swapneeta S. Date,

Current address: Oak Ridge National Laboratory, Oak Ridge, TN, United States of America

Affiliations Department of Cell Physiology and Molecular Biophysics, School of Medicine, Texas Tech University Health Sciences Center, Lubbock, TX, United States of America, Center for Membrane Protein Research, School of Medicine, Texas Tech University Health Sciences Center, Lubbock, TX, United States of America
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Mariana C. Fiori,

Affiliations Department of Cell Physiology and Molecular Biophysics, School of Medicine, Texas Tech University Health Sciences Center, Lubbock, TX, United States of America, Center for Membrane Protein Research, School of Medicine, Texas Tech University Health Sciences Center, Lubbock, TX, United States of America
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Guillermo A. Altenberg,

Affiliations Department of Cell Physiology and Molecular Biophysics, School of Medicine, Texas Tech University Health Sciences Center, Lubbock, TX, United States of America, Center for Membrane Protein Research, School of Medicine, Texas Tech University Health Sciences Center, Lubbock, TX, United States of America
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Michaela Jansen

* E-mail: Michaela.jansen@ttuhsc.edu

Affiliations Department of Cell Physiology and Molecular Biophysics, School of Medicine, Texas Tech University Health Sciences Center, Lubbock, TX, United States of America, Center for Membrane Protein Research, School of Medicine, Texas Tech University Health Sciences Center, Lubbock, TX, United States of America

http://orcid.org/0000-0003-1325-9700

⨯

Expression in Sf9 insect cells, purification and functional reconstitution of the human proton-coupled folate transporter (PCFT, SLC46A1)

Swapneeta S. Date,
Mariana C. Fiori,
Guillermo A. Altenberg,
Michaela Jansen

Published: May 11, 2017
https://doi.org/10.1371/journal.pone.0177572

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