Analysis of ParB-centromere interactions by multiplex SPR imaging reveals specific patterns for binding ParB in six centromeres of Burkholderiales chromosomes and plasmids

Flavien Pillet; Fanny Marie Passot; Franck Pasta; Véronique Anton Leberre; Jean-Yves Bouet

doi:10.1371/journal.pone.0177056

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Research Article

Analysis of ParB-centromere interactions by multiplex SPR imaging reveals specific patterns for binding ParB in six centromeres of Burkholderiales chromosomes and plasmids

Flavien Pillet,

Current address: Institut de Pharmacologie et de Biologie Structurale, Centre National de la Recherche Scientifique, Université Paul Sabatier, Toulouse, France

Affiliations Ingénierie des Systèmes Biologiques et des Procédés INRA UMR792, Institut National de la Recherche Agronomique, Institut National des Sciences Appliquées, Toulouse, France, Laboratoire d’Ingénierie des Systèmes Biologiques et des Procédés CNRS UMR5504, Centre National de la Recherche Scientifique, Institut National des Sciences Appliquées, Université de Toulouse, Toulouse, France
⨯
Fanny Marie Passot,

Current address: Molecular Cell Biology Unit, Biology Department, Lund University, Lund, Sweden

Affiliation Laboratoire de Microbiologie et Génétique Moléculaires, Centre de Biologie Intégrative, Centre National de la Recherche Scientifique, Université Paul Sabatier, Toulouse, France
⨯
Franck Pasta ,

* E-mail: pasta@ibcg.biotoul.fr

Affiliation Laboratoire de Microbiologie et Génétique Moléculaires, Centre de Biologie Intégrative, Centre National de la Recherche Scientifique, Université Paul Sabatier, Toulouse, France

http://orcid.org/0000-0003-4972-5494

⨯
Véronique Anton Leberre ,

Contributed equally to this work with: Véronique Anton Leberre, Jean-Yves Bouet

Affiliations Ingénierie des Systèmes Biologiques et des Procédés INRA UMR792, Institut National de la Recherche Agronomique, Institut National des Sciences Appliquées, Toulouse, France, Laboratoire d’Ingénierie des Systèmes Biologiques et des Procédés CNRS UMR5504, Centre National de la Recherche Scientifique, Institut National des Sciences Appliquées, Université de Toulouse, Toulouse, France
⨯
Jean-Yves Bouet

Contributed equally to this work with: Véronique Anton Leberre, Jean-Yves Bouet

Affiliation Laboratoire de Microbiologie et Génétique Moléculaires, Centre de Biologie Intégrative, Centre National de la Recherche Scientifique, Université Paul Sabatier, Toulouse, France
⨯

Analysis of ParB-centromere interactions by multiplex SPR imaging reveals specific patterns for binding ParB in six centromeres of Burkholderiales chromosomes and plasmids

Flavien Pillet,
Fanny Marie Passot,
Franck Pasta,
Véronique Anton Leberre,
Jean-Yves Bouet

Published: May 31, 2017
https://doi.org/10.1371/journal.pone.0177056

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Burkholderia cenocepacia
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Centromeres
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Nucleotides
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Plasmids
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Ralstonia
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Burkholderia
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Electrophoretic mobility shift assay
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