Modular Protein Expression Toolbox (MoPET), a standardized assembly system for defined expression constructs and expression optimization libraries

Open Access

Peer-reviewed

Research Article

Ernst Weber ,

* E-mail: ernst.weber@bayer.com

Affiliation Antibody Lead Discovery, Biologics Research, Bayer AG, Cologne, Germany

http://orcid.org/0000-0002-3743-3083

⨯
Jörg Birkenfeld,

Current address: R&D Biologics Research/Protein Therapeutics, Sanofi-Aventis Deutschland GmbH, Frankfurt am Main, Germany

Affiliation Antibody Lead Discovery, Biologics Research, Bayer AG, Cologne, Germany
⨯
Jürgen Franz,

Affiliation Cell and Protein Sciences, Biologics Research, Bayer AG, Wuppertal, Germany
⨯
Uwe Gritzan,

Affiliation Protein Engineering and Assays, Biologics Research, Bayer AG, Cologne, Germany
⨯
Lars Linden,

Affiliation Cell and Protein Sciences, Biologics Research, Bayer AG, Wuppertal, Germany
⨯
Mark Trautwein

Affiliation Cell and Protein Sciences, Biologics Research, Bayer AG, Wuppertal, Germany
⨯

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Cloning
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DNA cloning
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Plasmid construction
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Signal peptides
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Protein expression
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Library screening
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Polymerase chain reaction
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Optimization
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