An improved method to quantitate mature plant microRNA in biological matrices using modified periodate treatment and inclusion of internal controls

Open Access

Peer-reviewed

Research Article

Haiqiu Huang,

Affiliations Diet, Genomics and Immunology Laboratory, Beltsville Human Nutrition Research Center, USDA-ARS, Beltsville, Maryland, United States of America, Office of Dietary Supplements, NIH, Bethesda, Maryland, United States of America

http://orcid.org/0000-0003-1543-3901

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Jamin Roh,

Affiliation Diet, Genomics and Immunology Laboratory, Beltsville Human Nutrition Research Center, USDA-ARS, Beltsville, Maryland, United States of America
⨯
Cindy D. Davis,

Affiliation Office of Dietary Supplements, NIH, Bethesda, Maryland, United States of America
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Thomas T. Y. Wang

* E-mail: Tom.Wang@ars.usda.gov

Affiliation Diet, Genomics and Immunology Laboratory, Beltsville Human Nutrition Research Center, USDA-ARS, Beltsville, Maryland, United States of America
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MicroRNAs
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Oxidation
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Maize
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Methylation
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Diet
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Blood plasma
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RNA isolation
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Reverse transcription
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