Characterization and mutational analysis of a nicotinamide mononucleotide deamidase from Agrobacterium tumefaciens showing high thermal stability and catalytic efficiency

Ana Belén Martínez-Moñino; Rubén Zapata-Pérez; Antonio Ginés García-Saura; Fernando Gil-Ortiz; Manuela Pérez-Gilabert; Álvaro Sánchez-Ferrer

doi:10.1371/journal.pone.0174759

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Research Article

Characterization and mutational analysis of a nicotinamide mononucleotide deamidase from Agrobacterium tumefaciens showing high thermal stability and catalytic efficiency

Ana Belén Martínez-Moñino,

Affiliations Department of Biochemistry and Molecular Biology-A, Faculty of Biology, Regional Campus of International Excellence “Campus Mare Nostrum”, University of Murcia, Campus Espinardo, E-30100 MURCIA, Spain, Murcia Biomedical Research Institute (IMIB), Murcia, Spain
⨯
Rubén Zapata-Pérez,

Affiliations Department of Biochemistry and Molecular Biology-A, Faculty of Biology, Regional Campus of International Excellence “Campus Mare Nostrum”, University of Murcia, Campus Espinardo, E-30100 MURCIA, Spain, Murcia Biomedical Research Institute (IMIB), Murcia, Spain
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Antonio Ginés García-Saura,

Affiliations Department of Biochemistry and Molecular Biology-A, Faculty of Biology, Regional Campus of International Excellence “Campus Mare Nostrum”, University of Murcia, Campus Espinardo, E-30100 MURCIA, Spain, Murcia Biomedical Research Institute (IMIB), Murcia, Spain
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Fernando Gil-Ortiz,

Affiliation CELLS-ALBA Synchrotron Light Source, Barcelona, Spain
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Manuela Pérez-Gilabert,

Affiliations Department of Biochemistry and Molecular Biology-A, Faculty of Biology, Regional Campus of International Excellence “Campus Mare Nostrum”, University of Murcia, Campus Espinardo, E-30100 MURCIA, Spain, Murcia Biomedical Research Institute (IMIB), Murcia, Spain
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Álvaro Sánchez-Ferrer

* E-mail: alvaro@um.es

Affiliations Department of Biochemistry and Molecular Biology-A, Faculty of Biology, Regional Campus of International Excellence “Campus Mare Nostrum”, University of Murcia, Campus Espinardo, E-30100 MURCIA, Spain, Murcia Biomedical Research Institute (IMIB), Murcia, Spain

http://orcid.org/0000-0001-7266-4402

⨯

Characterization and mutational analysis of a nicotinamide mononucleotide deamidase from Agrobacterium tumefaciens showing high thermal stability and catalytic efficiency

Ana Belén Martínez-Moñino,
Rubén Zapata-Pérez,
Antonio Ginés García-Saura,
Fernando Gil-Ortiz,
Manuela Pérez-Gilabert,
Álvaro Sánchez-Ferrer

Published: April 7, 2017
https://doi.org/10.1371/journal.pone.0174759

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Agrobacterium tumefaciens
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Melting
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Mutational analysis
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Crystal structure
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Thermal stability
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Enzyme structure
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Catalysis
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Enzymes
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