Multiplex PCR as a tool for the diagnosis of Leishmania spp. kDNA and the gapdh housekeeping gene of mammal hosts

Open Access

Peer-reviewed

Research Article

Renata de Cássia-Pires,

Affiliation Laboratory of Trypanosomatid Biology, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Rio de Janeiro, Brazil
⨯
Myllena de Fátima Alheiros Dias de Melo,

Affiliation Laboratory of Molecular Biology and Endemic Diseases, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Rio de Janeiro, Brazil
⨯
Raquel da Hora Barbosa,

Affiliation Center of Medical Sciences, Biomedical Institute, Fluminense Federal University, UFF, Niterói, Rio de Janeiro, Brazil
⨯
André Luiz Rodrigues Roque

* E-mail: roque@ioc.fiocruz.br

Affiliation Laboratory of Trypanosomatid Biology, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Rio de Janeiro, Brazil
⨯

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Leishmania
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Polymerase chain reaction
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Kinetoplasts
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Mammals
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Sequence alignment
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Multiple alignment calculation
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Parasitic diseases
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DNA
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