Selection of internal reference genes for normalization of reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis in the rumen epithelium

Jose V. Die; Ransom L. Baldwin; Lisa J. Rowland; Robert Li; Sunghee Oh; Congjun Li; Erin E. Connor; Maria-Jose Ranilla

doi:10.1371/journal.pone.0172674

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Peer-reviewed

Research Article

Selection of internal reference genes for normalization of reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis in the rumen epithelium

Jose V. Die,

Current address: Departamento de Genética, University of Córdoba, Córdoba, Spain

Affiliation Genetic Improvement of Fruits and Vegetables Laboratory, US Department of Agriculture, Agricultural Research Service, Beltsville, Maryland, United States of America
⨯
Ransom L. Baldwin ,

* E-mail: ransom.baldwin@ars.usda.gov

Affiliation Animal Genomics and Improvement Laboratory, US Department of Agriculture, Agricultural Research Service, Beltsville, Maryland, United States of America

http://orcid.org/0000-0002-0753-4377

⨯
Lisa J. Rowland,

Affiliation Genetic Improvement of Fruits and Vegetables Laboratory, US Department of Agriculture, Agricultural Research Service, Beltsville, Maryland, United States of America
⨯
Robert Li,

Affiliation Animal Genomics and Improvement Laboratory, US Department of Agriculture, Agricultural Research Service, Beltsville, Maryland, United States of America
⨯
Sunghee Oh,

Affiliation Dept. of Computer Science & Statistics, Jeju National University, Jeju City, Jeju Do, S. Korea
⨯
Congjun Li,

Affiliation Animal Genomics and Improvement Laboratory, US Department of Agriculture, Agricultural Research Service, Beltsville, Maryland, United States of America
⨯
Erin E. Connor,

Affiliation Animal Genomics and Improvement Laboratory, US Department of Agriculture, Agricultural Research Service, Beltsville, Maryland, United States of America
⨯
Maria-Jose Ranilla

Affiliation Departamento de Producción Animal, Universidad de León, León, Spain
⨯

Selection of internal reference genes for normalization of reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis in the rumen epithelium

Jose V. Die,
Ransom L. Baldwin,
Lisa J. Rowland,
Robert Li,
Sunghee Oh,
Congjun Li,
Erin E. Connor,
Maria-Jose Ranilla

Published: February 24, 2017
https://doi.org/10.1371/journal.pone.0172674

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Gene expression
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