Bacterial expression, correct membrane targeting and functional folding of the HIV-1 membrane protein Vpu using a periplasmic signal peptide

Arpan Deb; William A. Johnson; Alexander P. Kline; Boston J. Scott; Lydia R. Meador; Dustin Srinivas; Jose M. Martin-Garcia; Katerina Dörner; Chad R. Borges; Rajeev Misra; Brenda G. Hogue; Petra Fromme; Tsafrir S. Mor

doi:10.1371/journal.pone.0172529

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Research Article

Bacterial expression, correct membrane targeting and functional folding of the HIV-1 membrane protein Vpu using a periplasmic signal peptide

Arpan Deb,

Current address: Department of Chemistry and Biochemistry, University of Montana, Missoula, Montana, United States of America

Affiliations School of Life Sciences, Arizona State University, Tempe, Arizona, United States of America, The Biodesign Center for Immunotherapy, Vaccines, and Virotherapy, The Biodesign Institute, Arizona State University, Tempe, Arizona, United States of America
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William A. Johnson,

Current address: School of Molecular Sciences, Arizona State University, Tempe, Arizona, United States of America

Affiliations School of Life Sciences, Arizona State University, Tempe, Arizona, United States of America, The Biodesign Center for Immunotherapy, Vaccines, and Virotherapy, The Biodesign Institute, Arizona State University, Tempe, Arizona, United States of America
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Alexander P. Kline,

Affiliations School of Life Sciences, Arizona State University, Tempe, Arizona, United States of America, The Biodesign Center for Immunotherapy, Vaccines, and Virotherapy, The Biodesign Institute, Arizona State University, Tempe, Arizona, United States of America
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Boston J. Scott,

Affiliations School of Life Sciences, Arizona State University, Tempe, Arizona, United States of America, The Biodesign Center for Immunotherapy, Vaccines, and Virotherapy, The Biodesign Institute, Arizona State University, Tempe, Arizona, United States of America
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Lydia R. Meador,

Current address: Department of Basic Medical Sciences, The University of Arizona College of Medicine Phoenix, Phoenix, Arizona, United States of America

Affiliations School of Life Sciences, Arizona State University, Tempe, Arizona, United States of America, The Biodesign Center for Immunotherapy, Vaccines, and Virotherapy, The Biodesign Institute, Arizona State University, Tempe, Arizona, United States of America
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Dustin Srinivas,

Current address: Department of Biomedical Sciences, Cedars Sinai Medical Center, Los Angeles, California, United States of America

Affiliations School of Life Sciences, Arizona State University, Tempe, Arizona, United States of America, The Biodesign Center for Immunotherapy, Vaccines, and Virotherapy, The Biodesign Institute, Arizona State University, Tempe, Arizona, United States of America
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Jose M. Martin-Garcia,

Affiliations School of Molecular Sciences, Arizona State University, Tempe, Arizona, United States of America, The Biodesign Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University, Tempe, Arizona, United States of America
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Katerina Dörner,

Current address: European XFEL GmbH, Schenefeld, Germany

Affiliation School of Molecular Sciences, Arizona State University, Tempe, Arizona, United States of America
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Chad R. Borges,

Affiliations School of Molecular Sciences, Arizona State University, Tempe, Arizona, United States of America, The Biodesign Center for Personal Diagnostics, The Biodesign Institute, Arizona State University, Tempe, Arizona, United States of America
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Rajeev Misra,

Affiliation School of Life Sciences, Arizona State University, Tempe, Arizona, United States of America
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Brenda G. Hogue,

Affiliations School of Life Sciences, Arizona State University, Tempe, Arizona, United States of America, The Biodesign Center for Immunotherapy, Vaccines, and Virotherapy, The Biodesign Institute, Arizona State University, Tempe, Arizona, United States of America, The Biodesign Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University, Tempe, Arizona, United States of America
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Petra Fromme,

Affiliations School of Molecular Sciences, Arizona State University, Tempe, Arizona, United States of America, The Biodesign Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University, Tempe, Arizona, United States of America
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Tsafrir S. Mor

* E-mail: tsafrir.mor@asu.edu

Affiliations School of Life Sciences, Arizona State University, Tempe, Arizona, United States of America, The Biodesign Center for Immunotherapy, Vaccines, and Virotherapy, The Biodesign Institute, Arizona State University, Tempe, Arizona, United States of America

http://orcid.org/0000-0002-6123-6194

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Bacterial expression, correct membrane targeting and functional folding of the HIV-1 membrane protein Vpu using a periplasmic signal peptide

Arpan Deb,
William A. Johnson,
Alexander P. Kline,
Boston J. Scott,
Lydia R. Meador,
Dustin Srinivas,
Jose M. Martin-Garcia,
Katerina Dörner,
Chad R. Borges,
Rajeev Misra

Published: February 22, 2017
https://doi.org/10.1371/journal.pone.0172529

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DNA-binding proteins
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