SplitAx: A novel method to assess the function of engineered nucleases

Open Access

Peer-reviewed

Research Article

Richard A. Axton,

Affiliation MRC Centre for Regenerative Medicine, SCRM Building, The University of Edinburgh, Edinburgh bioQuarter, 5 Little France Drive, Edinburgh, United Kingdom
⨯
Sharmin S. Haideri,

Affiliation MRC Centre for Regenerative Medicine, SCRM Building, The University of Edinburgh, Edinburgh bioQuarter, 5 Little France Drive, Edinburgh, United Kingdom
⨯
Martha Lopez-Yrigoyen,

Affiliation MRC Centre for Regenerative Medicine, SCRM Building, The University of Edinburgh, Edinburgh bioQuarter, 5 Little France Drive, Edinburgh, United Kingdom
⨯
Helen A. Taylor,

Affiliation MRC Centre for Regenerative Medicine, SCRM Building, The University of Edinburgh, Edinburgh bioQuarter, 5 Little France Drive, Edinburgh, United Kingdom
⨯
Lesley M. Forrester

* E-mail: L.Forrester@ed.ac.uk

Affiliation MRC Centre for Regenerative Medicine, SCRM Building, The University of Edinburgh, Edinburgh bioQuarter, 5 Little France Drive, Edinburgh, United Kingdom
⨯

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Polymerase chain reaction
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Cloning
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Transfection
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Genomics
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Zinc finger nucleases
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Mammalian genomics
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Vector cloning
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Non-homologous end joining
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