Phosphorylated fraction of H2AX as a measurement for DNA damage in cancer cells and potential applications of a novel assay

Jiuping Ji; Yiping Zhang; Christophe E. Redon; William C. Reinhold; Alice P. Chen; Laura K. Fogli; Susan L. Holbeck; Ralph E. Parchment; Melinda Hollingshead; Joseph E. Tomaszewski; Quentin Dudon; Yves Pommier; James H. Doroshow; William M. Bonner

doi:10.1371/journal.pone.0171582

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Open Access

Peer-reviewed

Research Article

Phosphorylated fraction of H2AX as a measurement for DNA damage in cancer cells and potential applications of a novel assay

Jiuping Ji ,

* E-mail: jijiupi@mail.nih.gov

Affiliation Applied/Developmental Research Directorate, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, Frederick, Maryland, United States of America
⨯
Yiping Zhang,

Affiliation Applied/Developmental Research Directorate, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, Frederick, Maryland, United States of America
⨯
Christophe E. Redon,

Affiliation Developmental Therapeutics Branch, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland, United States of America
⨯
William C. Reinhold,

Affiliation Developmental Therapeutics Branch, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland, United States of America
⨯
Alice P. Chen,

Affiliation Early Clinical Trials Development Program, Division of Cancer Treatment and Diagnosis, National Cancer Institute, Bethesda, Maryland, United States of America
⨯
Laura K. Fogli ,

¶‡ Author is a government contractor, not a federal employee.

Affiliation Division of Cancer Treatment and Diagnosis, National Cancer Institute, Bethesda, Maryland, United States of America
⨯
Susan L. Holbeck,

Affiliation Division of Cancer Treatment and Diagnosis, National Cancer Institute, Bethesda, Maryland, United States of America
⨯
Ralph E. Parchment,

Affiliation Applied/Developmental Research Directorate, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, Frederick, Maryland, United States of America
⨯
Melinda Hollingshead,

Affiliation Division of Cancer Treatment and Diagnosis, National Cancer Institute, Bethesda, Maryland, United States of America
⨯
Joseph E. Tomaszewski,

Affiliation Division of Cancer Treatment and Diagnosis, National Cancer Institute, Bethesda, Maryland, United States of America
⨯
Quentin Dudon,

Affiliation Developmental Therapeutics Branch, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland, United States of America
⨯
Yves Pommier,

Affiliation Developmental Therapeutics Branch, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland, United States of America
⨯
James H. Doroshow,

Affiliations Developmental Therapeutics Branch, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland, United States of America, Division of Cancer Treatment and Diagnosis, National Cancer Institute, Bethesda, Maryland, United States of America
⨯
William M. Bonner

Affiliation Developmental Therapeutics Branch, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland, United States of America
⨯

Phosphorylated fraction of H2AX as a measurement for DNA damage in cancer cells and potential applications of a novel assay

Jiuping Ji,
Yiping Zhang,
Christophe E. Redon,
William C. Reinhold,
Alice P. Chen,
Laura K. Fogli,
Susan L. Holbeck,
Ralph E. Parchment,
Melinda Hollingshead,
Joseph E. Tomaszewski

Published: February 3, 2017
https://doi.org/10.1371/journal.pone.0171582

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Enzyme-linked immunoassays
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DNA damage
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Cancer treatment
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Cancers and neoplasms
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Malignant tumors
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Phosphorylation
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Drug therapy
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Histones
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