Engineering of mCherry variants with long Stokes shift, red-shifted fluorescence, and low cytotoxicity

Open Access

Peer-reviewed

Research Article

Yi Shen,

Affiliation Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada
⨯
Yingche Chen,

Affiliation Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada
⨯
Jiahui Wu,

Affiliation Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada
⨯
Nathan C. Shaner,

Affiliation Department of Photobiology and Bioimaging, The Scintillon Institute, San Diego, California, United States of America
⨯
Robert E. Campbell

* E-mail: robert.e.campbell@ualberta.ca

Affiliation Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada
⨯

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Fluorescence
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Fluorescence imaging
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Chromophores
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Directed evolution
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Mutagenesis
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Cytotoxicity assay
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