Highly sensitive detection of a HER2 12-base pair duplicated insertion mutation in lung cancer using the Eprobe-PCR method

Yoshiaki Takase; Kengo Usui; Kimihiro Shimizu; Yasumasa Kimura; Tatsuo Ichihara; Takahiro Ohkawa; Jun Atsumi; Yasuaki Enokida; Seshiru Nakazawa; Kai Obayashi; Yoichi Ohtaki; Toshiteru Nagashima; Yasumasa Mitani; Izumi Takeyoshi

doi:10.1371/journal.pone.0171225

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Open Access

Peer-reviewed

Research Article

Highly sensitive detection of a HER2 12-base pair duplicated insertion mutation in lung cancer using the Eprobe-PCR method

Yoshiaki Takase,

Affiliation Department of Thoracic and Visceral Organ Surgery, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan
⨯
Kengo Usui ,

* E-mail: kengo.usui@riken.jp

Affiliation Division of Genomic Technologies, RIKEN Center of Life Science Technologies, Yokohama, Kanagawa, Japan
⨯
Kimihiro Shimizu,

Affiliation Department of Thoracic and Visceral Organ Surgery, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan
⨯
Yasumasa Kimura,

Current address: Division of Systems Immunology, Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo, Japan

Affiliations Division of Genomic Technologies, RIKEN Center of Life Science Technologies, Yokohama, Kanagawa, Japan, K.K. DNAFORM, Yokohama, Kanagawa, Japan
⨯
Tatsuo Ichihara,

Affiliation K.K. DNAFORM, Yokohama, Kanagawa, Japan
⨯
Takahiro Ohkawa,

Affiliation Division of Genomic Technologies, RIKEN Center of Life Science Technologies, Yokohama, Kanagawa, Japan
⨯
Jun Atsumi,

Affiliation Department of Thoracic and Visceral Organ Surgery, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan
⨯
Yasuaki Enokida,

Affiliation Department of Thoracic and Visceral Organ Surgery, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan
⨯
Seshiru Nakazawa,

Affiliation Department of Thoracic and Visceral Organ Surgery, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan
⨯
Kai Obayashi,

Affiliation Department of Thoracic and Visceral Organ Surgery, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan
⨯
Yoichi Ohtaki,

Affiliation Department of Thoracic and Visceral Organ Surgery, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan
⨯
Toshiteru Nagashima,

Affiliation Department of Thoracic and Visceral Organ Surgery, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan
⨯
Yasumasa Mitani,

Affiliation K.K. DNAFORM, Yokohama, Kanagawa, Japan
⨯
Izumi Takeyoshi

Affiliation Department of Thoracic and Visceral Organ Surgery, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan
⨯

Highly sensitive detection of a HER2 12-base pair duplicated insertion mutation in lung cancer using the Eprobe-PCR method

Yoshiaki Takase,
Kengo Usui,
Kimihiro Shimizu,
Yasumasa Kimura,
Tatsuo Ichihara,
Takahiro Ohkawa,
Jun Atsumi,
Yasuaki Enokida,
Seshiru Nakazawa,
Kai Obayashi

Published: February 2, 2017
https://doi.org/10.1371/journal.pone.0171225

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Insertion mutation
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Lung and intrathoracic tumors
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Mutation detection
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Polymerase chain reaction
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Point mutation
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Adenocarcinoma
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Next-generation sequencing
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