A Split-GFP Gateway Cloning System for Topology Analyses of Membrane Proteins in Plants

Open Access

Peer-reviewed

Research Article

Wenjun Xie,

Affiliation Plant Defence Genetics, Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, Frederiksberg C, Denmark
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Mads Eggert Nielsen,

Affiliation Plant Defence Genetics, Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, Frederiksberg C, Denmark
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Carsten Pedersen,

Affiliation Plant Defence Genetics, Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, Frederiksberg C, Denmark
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Hans Thordal-Christensen

* E-mail: htc@plen.ku.dk

Affiliation Plant Defence Genetics, Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, Frederiksberg C, Denmark
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Membrane proteins
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Cell membranes
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Endoplasmic reticulum
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Green fluorescent protein
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Plasmid construction
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Confocal microscopy
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Membrane receptor signaling
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Nicotiana
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