Active Hydrogen Bond Network (AHBN) and Applications for Improvement of Thermal Stability and pH-Sensitivity of Pullulanase from Bacillus naganoensis

Qing-Yan Wang; Neng-Zhong Xie; Qi-Shi Du; Yan Qin; Jian-Xiu Li; Jian-Zong Meng; Ri-Bo Huang

doi:10.1371/journal.pone.0169080

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Peer-reviewed

Research Article

Active Hydrogen Bond Network (AHBN) and Applications for Improvement of Thermal Stability and pH-Sensitivity of Pullulanase from Bacillus naganoensis

Qing-Yan Wang ,

Contributed equally to this work with: Qing-Yan Wang, Neng-Zhong Xie

Affiliation State Key Laboratory of Biomass Enzyme Technology, National Engineering Research Center for Non-Food Biorefinery, Guangxi Academy of Sciences, Nanning, Guangxi, China
⨯
Neng-Zhong Xie ,

Contributed equally to this work with: Qing-Yan Wang, Neng-Zhong Xie

Affiliation State Key Laboratory of Biomass Enzyme Technology, National Engineering Research Center for Non-Food Biorefinery, Guangxi Academy of Sciences, Nanning, Guangxi, China
⨯
Qi-Shi Du ,

* E-mail: duqishi@foxmail.com

Affiliations State Key Laboratory of Biomass Enzyme Technology, National Engineering Research Center for Non-Food Biorefinery, Guangxi Academy of Sciences, Nanning, Guangxi, China, Gordon Life Science Institute, Belmont, MA, United States of America
⨯
Yan Qin,

Affiliation State Key Laboratory of Biomass Enzyme Technology, National Engineering Research Center for Non-Food Biorefinery, Guangxi Academy of Sciences, Nanning, Guangxi, China
⨯
Jian-Xiu Li,

Affiliations State Key Laboratory of Biomass Enzyme Technology, National Engineering Research Center for Non-Food Biorefinery, Guangxi Academy of Sciences, Nanning, Guangxi, China, Life Science and Technology College, Guangxi University, Nanning, Guangxi, China
⨯
Jian-Zong Meng,

Affiliation Life Science and Technology College, Guangxi University, Nanning, Guangxi, China
⨯
Ri-Bo Huang

Affiliations State Key Laboratory of Biomass Enzyme Technology, National Engineering Research Center for Non-Food Biorefinery, Guangxi Academy of Sciences, Nanning, Guangxi, China, Life Science and Technology College, Guangxi University, Nanning, Guangxi, China
⨯

Active Hydrogen Bond Network (AHBN) and Applications for Improvement of Thermal Stability and pH-Sensitivity of Pullulanase from Bacillus naganoensis

Qing-Yan Wang,
Neng-Zhong Xie,
Qi-Shi Du,
Yan Qin,
Jian-Xiu Li,
Jian-Zong Meng,
Ri-Bo Huang

Published: January 19, 2017
https://doi.org/10.1371/journal.pone.0169080

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