Structural and Catalytic Properties of S1 Nuclease from Aspergillus oryzae Responsible for Substrate Recognition, Cleavage, Non–Specificity, and Inhibition

Tomáš Kovaľ; Lars H. Østergaard; Jan Lehmbeck; Allan Nørgaard; Petra Lipovová; Jarmila Dušková; Tereza Skálová; Mária Trundová; Petr Kolenko; Karla Fejfarová; Jan Stránský; Leona Švecová; Jindřich Hašek; Jan Dohnálek

doi:10.1371/journal.pone.0168832

Loading metrics

Open Access

Peer-reviewed

Research Article

Structural and Catalytic Properties of S1 Nuclease from Aspergillus oryzae Responsible for Substrate Recognition, Cleavage, Non–Specificity, and Inhibition

Tomáš Kovaľ ,

* E-mail: koval.tomas@gmail.com (TK); jan.dohnalek@ibt.cas.cz (JD)

Affiliation Laboratory of Structure and Function of Biomolecules, Institute of Biotechnology CAS, v. v. i., Biocev, Vestec, Czech Republic
⨯
Lars H. Østergaard,

Affiliation Department of Agile Protein Screening, Novozymes A/S, Bagsvaerd, Denmark
⨯
Jan Lehmbeck,

Affiliation Department of Fungal Strain Technology, Novozymes A/S, Bagsvaerd, Denmark
⨯
Allan Nørgaard,

Affiliation Department of Protein Biochemistry and Stability, Novozymes A/S, Bagsvaerd, Denmark
⨯
Petra Lipovová,

Affiliation Department of Biochemistry and Microbiology, University of Chemistry and Technology, Prague, Czech Republic
⨯
Jarmila Dušková,

Affiliation Laboratory of Structure and Function of Biomolecules, Institute of Biotechnology CAS, v. v. i., Biocev, Vestec, Czech Republic
⨯
Tereza Skálová,

Affiliation Laboratory of Structure and Function of Biomolecules, Institute of Biotechnology CAS, v. v. i., Biocev, Vestec, Czech Republic
⨯
Mária Trundová,

Affiliation Laboratory of Structure and Function of Biomolecules, Institute of Biotechnology CAS, v. v. i., Biocev, Vestec, Czech Republic
⨯
Petr Kolenko,

Affiliation Laboratory of Structure and Function of Biomolecules, Institute of Biotechnology CAS, v. v. i., Biocev, Vestec, Czech Republic
⨯
Karla Fejfarová,

Affiliation Laboratory of Structure and Function of Biomolecules, Institute of Biotechnology CAS, v. v. i., Biocev, Vestec, Czech Republic
⨯
Jan Stránský,

Affiliation Laboratory of Structure and Function of Biomolecules, Institute of Biotechnology CAS, v. v. i., Biocev, Vestec, Czech Republic
⨯
Leona Švecová,

Affiliation Laboratory of Structure and Function of Biomolecules, Institute of Biotechnology CAS, v. v. i., Biocev, Vestec, Czech Republic
⨯
Jindřich Hašek,

Affiliation Laboratory of Structure and Function of Biomolecules, Institute of Biotechnology CAS, v. v. i., Biocev, Vestec, Czech Republic
⨯
Jan Dohnálek

* E-mail: koval.tomas@gmail.com (TK); jan.dohnalek@ibt.cas.cz (JD)

Affiliation Laboratory of Structure and Function of Biomolecules, Institute of Biotechnology CAS, v. v. i., Biocev, Vestec, Czech Republic
⨯

Structural and Catalytic Properties of S1 Nuclease from Aspergillus oryzae Responsible for Substrate Recognition, Cleavage, Non–Specificity, and Inhibition

Tomáš Kovaľ,
Lars H. Østergaard,
Jan Lehmbeck,
Allan Nørgaard,
Petra Lipovová,
Jarmila Dušková,
Tereza Skálová,
Mária Trundová,
Petr Kolenko,
Karla Fejfarová

Published: December 30, 2016
https://doi.org/10.1371/journal.pone.0168832

Reader Comments (0)

Post a new comment on this article

Subject Areas
?

For more information about PLOS Subject Areas, click here.
We want your feedback. Do these Subject Areas make sense for this article? Click the target next to the incorrect Subject Area and let us know. Thanks for your help!

Nucleases
Is the Subject Area "Nucleases" applicable to this article?

Thanks for your feedback.
Phosphates
Is the Subject Area "Phosphates" applicable to this article?

Thanks for your feedback.
Crystal structure
Is the Subject Area "Crystal structure" applicable to this article?

Thanks for your feedback.
Zinc
Is the Subject Area "Zinc" applicable to this article?

Thanks for your feedback.
RNA structure
Is the Subject Area "RNA structure" applicable to this article?

Thanks for your feedback.
Crystallization
Is the Subject Area "Crystallization" applicable to this article?

Thanks for your feedback.
Nucleobases
Is the Subject Area "Nucleobases" applicable to this article?

Thanks for your feedback.
Nucleotides
Is the Subject Area "Nucleotides" applicable to this article?

Thanks for your feedback.