Rapid Generation of Marker-Free P. falciparum Fluorescent Reporter Lines Using Modified CRISPR/Cas9 Constructs and Selection Protocol

Catherin Marin Mogollon; Fiona J. A. van Pul; Takashi Imai; Jai Ramesar; Séverine Chevalley-Maurel; Guido M. de Roo; Sabrina A. J. Veld; Hans Kroeze; Blandine M. D. Franke-Fayard; Chris J. Janse; Shahid M. Khan

doi:10.1371/journal.pone.0168362

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Open Access

Peer-reviewed

Research Article

Rapid Generation of Marker-Free P. falciparum Fluorescent Reporter Lines Using Modified CRISPR/Cas9 Constructs and Selection Protocol

Catherin Marin Mogollon,

Affiliation Leiden Malaria Research Group, Department of Parasitology, Leiden University Medical Center (LUMC), Leiden, The Netherlands
⨯
Fiona J. A. van Pul,

Affiliation Leiden Malaria Research Group, Department of Parasitology, Leiden University Medical Center (LUMC), Leiden, The Netherlands
⨯
Takashi Imai,

Affiliation Leiden Malaria Research Group, Department of Parasitology, Leiden University Medical Center (LUMC), Leiden, The Netherlands
⨯
Jai Ramesar,

Affiliation Leiden Malaria Research Group, Department of Parasitology, Leiden University Medical Center (LUMC), Leiden, The Netherlands
⨯
Séverine Chevalley-Maurel,

Affiliation Leiden Malaria Research Group, Department of Parasitology, Leiden University Medical Center (LUMC), Leiden, The Netherlands
⨯
Guido M. de Roo,

Affiliation Department of Hematology, Leiden University Medical Center (LUMC), Leiden, The Netherlands
⨯
Sabrina A. J. Veld,

Affiliation Department of Hematology, Leiden University Medical Center (LUMC), Leiden, The Netherlands
⨯
Hans Kroeze,

Affiliation Leiden Malaria Research Group, Department of Parasitology, Leiden University Medical Center (LUMC), Leiden, The Netherlands
⨯
Blandine M. D. Franke-Fayard,

Affiliation Leiden Malaria Research Group, Department of Parasitology, Leiden University Medical Center (LUMC), Leiden, The Netherlands
⨯
Chris J. Janse,

Affiliation Leiden Malaria Research Group, Department of Parasitology, Leiden University Medical Center (LUMC), Leiden, The Netherlands
⨯
Shahid M. Khan

* E-mail: S.M.Khan@lumc.nl

Affiliation Leiden Malaria Research Group, Department of Parasitology, Leiden University Medical Center (LUMC), Leiden, The Netherlands

http://orcid.org/0000-0002-0533-4670

⨯

Rapid Generation of Marker-Free P. falciparum Fluorescent Reporter Lines Using Modified CRISPR/Cas9 Constructs and Selection Protocol

Catherin Marin Mogollon,
Fiona J. A. van Pul,
Takashi Imai,
Jai Ramesar,
Séverine Chevalley-Maurel,
Guido M. de Roo,
Sabrina A. J. Veld,
Hans Kroeze,
Blandine M. D. Franke-Fayard,
Chris J. Janse

Published: December 20, 2016
https://doi.org/10.1371/journal.pone.0168362

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