Off Target, but Sequence-Specific, shRNA-Associated Trans-Activation of Promoter Reporters in Transient Transfection Assays

Tomohiro Masuda; Jun Wan; Anitha Yerrabelli; Cindy Berlinicke; Alyssa Kallman; Jiang Qian; Donald J. Zack

doi:10.1371/journal.pone.0167867

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Peer-reviewed

Research Article

Off Target, but Sequence-Specific, shRNA-Associated Trans-Activation of Promoter Reporters in Transient Transfection Assays

Tomohiro Masuda,

Affiliation Department of Ophthalmology, Wilmer Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America

http://orcid.org/0000-0002-6668-1246

⨯
Jun Wan,

Affiliation Department of Ophthalmology, Wilmer Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America
⨯
Anitha Yerrabelli,

Affiliation Department of Ophthalmology, Wilmer Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America
⨯
Cindy Berlinicke,

Affiliation Department of Ophthalmology, Wilmer Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America
⨯
Alyssa Kallman,

Affiliation Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America
⨯
Jiang Qian,

Affiliation Department of Ophthalmology, Wilmer Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America
⨯
Donald J. Zack

* E-mail: donzack@gmail.com

Affiliations Department of Ophthalmology, Wilmer Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America, Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America, Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America, Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America, Institut de la Vision, University Pierre and Marie Curie, Paris, France
⨯

Off Target, but Sequence-Specific, shRNA-Associated Trans-Activation of Promoter Reporters in Transient Transfection Assays

Tomohiro Masuda,
Jun Wan,
Anitha Yerrabelli,
Cindy Berlinicke,
Alyssa Kallman,
Jiang Qian,
Donald J. Zack

Published: December 15, 2016
https://doi.org/10.1371/journal.pone.0167867

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Transfection
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MicroRNAs
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Gene expression
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Luciferase
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Interferons
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Transcriptional control
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Transient transfection assay
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Microarrays
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